FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Atf3UAS.cSa
Open Close
General Information
Symbol
Dmel\Atf3UAS.cSa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Sekyrova
FlyBase ID
FBal0245992
Feature type
allele
Associated gene
Dmel\Atf3
Associated Insertion(s)
Carried in Construct
P{UAS-Atf3.S}
Also Known As
UAS-Atf3, UAS-atf3wt
Key Links
Transgenic product class
wild_type
(Sekyrova et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Sekyrova et al., 2010)
Mutagen
in vitro construct
(Sekyrova et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-Atf3.S}
Cytology
Description

UASt regulatory sequences drive expression of the Atf3 open reading frame.

(Sekyrova et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Atf3
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Larvae expressing Atf3Scer\UAS.cSa under the control of Scer\GAL4C7 have lipid droplets of reduced size in the fat body and reduced triacylglycerol levels.

      (Rynes et al., 2012)

      Expression of Atf3Scer\UAS.cSa under the control of Scer\GAL4GMR.PF results in flies with smaller eyes than normal, and the eyes have a glossy appearance.

      Animals expressing Atf3Scer\UAS.cSa under the control of Scer\GAL4ppl.PP develop normally until the pupal stage, but they usually (86%) die as pharate adults inside the puparium with a dorsally open cleft in the abdominal cuticle. Those mutant adults that do eclose show abdominal lesions filled with the old pupal cuticle and lacking adult pigmentation and bristles, often with a clot covering a bleeding wound. This defect can be partially or completely suppressed by supplementing mid-third instar larvae with dietary 20-hydroxyecdysone.

      Histoblast nests spread in the abdomens of pupae expressing Atf3Scer\UAS.cSa under the control of Scer\GAL4ppl.PP (as occurs in wild type), and at 16 hours after puparium formation (APF) they are comprised of a normal number of histoblasts. The histoblasts also differentiate the adult cuticle with sensory bristles in the mutant pupae, although polarity of the bristles near the dorsal cleft is abnormal. However, in contrast to controls, a large population of larval epidermal cells (LECs) remain in the dorsal abdomen at 48 hours APF in the mutant animals. These LECs persist throughout metamorphosis to the adult stage.

      Expression of Atf3Scer\UAS.cSa under the control of Scer\GAL4arm.PS results in adults with a dorsal cleft in the abdominal cuticle.

      Animals expressing Atf3Scer\UAS.cSa under the control of Scer\GAL4Eip71CD.657 usually die as pupae which lack adult cuticle, but occasional adult escapers, which have a dorsal cleft in the abdominal cuticle, are seen.

      Expression of Atf3Scer\UAS.cSa under the control of Scer\GAL4Lsp2.PH, Scer\GAL4T155 or Scer\GAL4Cg.PA does not result in abdominal defects.

      Expression of Atf3Scer\UAS.cSa under the control of Scer\GAL4Act5C.PI in clones in the mid-third larval instar (which results in expression in the polyploid larval cells but not in the diploid histoblasts) invariably results in a cleft in the abdominal cuticle of the resulting adults. The lateral and ventral parts of the abdomen, as well as the dorsal part, may be affected in these flies. Clones of larval epidermal cells which are expressing Atf3Scer\UAS.cSa under the control of Scer\GAL4Act5C.PI show membrane interdigitation at 18 and 24 hours after puparium formation (neighbouring wild-type cells have smooth membranes). These mutant cells can apically constrict, but they continue to adhere to the epithelium and so are not extruded from the epidermis, in contrast to wild-type cells.

      (Sekyrova et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Statement
      Reference

      Atf3UAS.cSa, Scer\GAL4ppl.PP is an enhancer of adult abdomen | dorsal phenotype of Rho1GD4726, Scer\GAL4ppl.PP

      (Sekyrova et al., 2010)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Atf3Scer\UAS.cSa under the control of Scer\GAL4Myo31DF-NP0001 suppresses the decrease in lipid levels seen in the intestines of p38c7B1 mutant flies.

      (Chakrabarti et al., 2014)

      The mutant eye phenotype caused by expression of Atf3Scer\UAS.cSa under the control of Scer\GAL4GMR.PF is completely suppressed by co-expression of JradsRNA.Scer\UAS, but is not suppressed by co-expression of kaydsRNA.Scer\UAS.

      The mutant eye phenotype caused by expression of Atf3Scer\UAS.cSa under the control of Scer\GAL4GMR.PF is exacerbated by co-expression of JraScer\UAS.cEa.

      The mutant eye phenotype caused by expression of Atf3Scer\UAS.cSa under the control of Scer\GAL4GMR.PF is is completely suppressed by co-expression of kayScer\UAS.cEa.

      Co-expression of Atf3Scer\UAS.cSa and Rho1GD4726 under the control of Scer\GAL4ppl.PP results in a more severe phenotype than co-expression of either construct alone; no animals eclose and the defect in the dorsal abdomen becomes more severe.

      Co-expression of Rho1V14.Scer\UAS suppresses the dorsal cleft in the abdominal cuticle caused by expression of Atf3Scer\UAS.cSa under the control of Scer\GAL4ppl.PP.

      Co-expression of JradsRNA.Scer\UAS restores viability in animals expressing Atf3Scer\UAS.cSa under the control of Scer\GAL4ppl.PP and 87% of the rescued adults eclose with a completely normal abdomen.

      Co-expression of kaydsRNA.Scer\UAS does not suppress the dorsal cleft in the abdominal cuticle caused by expression of Atf3Scer\UAS.cSa under the control of Scer\GAL4ppl.PP.

      (Sekyrova et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Atf3UAS.cSa/Scer\GAL4arm.PS rescues Atf376

      (Sekyrova et al., 2010)
      Comments

      Atf3Scer\UAS.cSa can rescue the lethality of Atf376/Y males or can reduce survival to below that of Atf376/Y males when expressed under the control of either Scer\GAL4fat or Scer\GAL4C7, depending on the insertion line used.

      Atf3Scer\UAS.cSa in the absence of a Scer\GAL4 driver rescues the abnormally large lipid droplets and high triacylglycerol levels seen in Atf376 larvae.

      (Rynes et al., 2012)

      Some Atf376 hemizygotes are rescued to adulthood by expression of Atf3Scer\UAS.cSa under the control of Scer\GAL4arm.PS.

      (Sekyrova et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Atf3Scer\UAS.cSa
      Atf3UAS.cSa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Sekyrova
      Secondary FlyBase IDs
        References (7)