FlyBase Allele Report: Dmel\Pten[5]

General Information

Symbol

Dmel\Pten⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0318138

Feature type

allele

Associated gene

Dmel\Pten

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Mensah et al., 2015)

Progenitor genotype

Cytology

Description

A single A to G (GAC to GGC) point mutation was identified at position 509 of the Pten open reading frame, which causes an aspartate (Asp) to glycine (Gly) substitution at position 170 of the Pten polypeptide.

(Mensah et al., 2015)

Amino acid replacement: D170G.

(Mensah et al., 2015)

Nucleotide substitution: A509G.

(Mensah et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:10,258,825..10,258,825 [-]

Nucleotide change:

A10258825G

Reported nucleotide change:

A509G

Amino acid change:

Reported amino acid change:

D170G

Comment:

Position of mutation on genomic sequence inferred by FlyBase curator from the reported nucleic acid and amino acid changes.

(Mensah et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior (with Pten¹)

(Mensah et al., 2015)

abnormal osmotic stress response (with Pten^dj189)

(Mensah et al., 2015)

abnormal oxidative stress response (with Pten^dj189)

(Mensah et al., 2015)

abnormal starvation stress response (with Pten^dj189)

(Mensah et al., 2015)

flightless (with Pten³)

(Mensah et al., 2015)

flightless (with Pten^dj189)

(Mensah et al., 2015)

flightless | progressive (with Pten¹)

(Mensah et al., 2015)

increased body size | male limited (with Pten¹)

(Mensah et al., 2015)

visible

(Mensah et al., 2015)

visible (with Df(2L)flp170B)

(Mensah et al., 2015)

visible (with Pten¹)

(Mensah et al., 2015)

visible (with Pten^dj189)

(Mensah et al., 2015)

Phenotype Manifest In

eye

(Mensah et al., 2015)

eye (with Pten¹)

(Mensah et al., 2015)

eye (with Pten^dj189)

(Mensah et al., 2015)

indirect flight muscle cell (with Pten¹)

(Mensah et al., 2015)

mitochondrion (with Pten¹)

(Mensah et al., 2015)

ommatidium (with Df(2L)flp170B)

(Mensah et al., 2015)

wing (with Pten¹)

(Mensah et al., 2015)

Detailed Description

Statement

Reference

Flies trans-heterozygous for Pten⁵ and Df(2L)flp170B have eyes with a slightly disorganised ommatidial array.

Homozygous Pten⁵ flies also display a rough eye phenotype.

Pten⁵/Pten¹ flies exhibit a highly penetrant, but mild, disorganised eye phenotype at the posterior edge in the midline.

Pten⁵/Pten^dj189 flies exhibit a highly penetrant, but mild, disorganised eye phenotype at the posterior edge in the midline.

Transheterozygous mutant Pten⁵/Pten¹ males have significantly (~20%) greater body mass than wild-type.

A small proportion of newly eclosed Pten⁵/Pten¹ flies display outstretched and paralysed wing phenotype. The flies are unable to fly. The proportion of flightless mutant increases as flies age.

(Mensah et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pten¹/Pten⁵ has flightless | progressive phenotype, suppressible | partially by Buffy^UAS.cQa/Scer\GAL4^arm.PS

(Mensah et al., 2015)

Pten¹/Pten⁵ has flightless | progressive phenotype, suppressible | partially by Buffy^UAS.cQa/Scer\GAL4^how-24B

(Mensah et al., 2015)

Pten¹/Pten⁵ has flightless | progressive phenotype, suppressible | partially by Rheb[+]/Rheb^AV4

(Mensah et al., 2015)

Pten¹/Pten⁵ has flightless | progressive phenotype, suppressible | partially by Tor[+]/mTor^ΔP

(Mensah et al., 2015)

Pten³/Pten⁵ has flightless phenotype, suppressible | partially by Akt1[+]/Akt¹

(Mensah et al., 2015)

Pten^dj189/Pten⁵ has flightless phenotype, suppressible | partially by Akt1[+]/Akt³

(Mensah et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Pten¹/Pten⁵ is partially rescued by Pten^+t6

(Mensah et al., 2015)

Pten^dj189/Pten⁵ is partially rescued by Pten^+t6

(Mensah et al., 2015)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Pten⁵

(Mensah et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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