FlyBase Allele Report: Dmel\path[dg50]

General Information

Symbol

Dmel\path^dg50

Species

D. melanogaster

Name

dendrite growth defective 50

FlyBase ID

FBal0305310

Feature type

allele

Associated gene

Dmel\path

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Lin et al., 2015)

Mutagen

ethyl methanesulfonate

(Lin et al., 2015)

Progenitor genotype

Cytology

Description

A 172-nucleotide deletion in path that causes a frameshift and premature stop, truncating the transmembrane domains. This should render the encoded protein nonfunctional.

(Lin et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:9,496,811..9,496,982 [-]

Comment:

A 172-nucleotide deletion in path that causes a frameshift and premature stop.

(Lin and Parrish, 2016.1.15)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal dorsal multidendritic neuron ddaA

(Lin et al., 2015)

abdominal dorsal multidendritic neuron ddaC

(Lin et al., 2015)

abdominal dorsal multidendritic neuron ddaC (with Df(3L)BSC773)

(Lin et al., 2015)

abdominal dorsal multidendritic neuron ddaC (with path^KG06640)

(Lin et al., 2015)

abdominal dorsal multidendritic neuron ddaC | germline clone

(Lin et al., 2015)

abdominal dorsal multidendritic neuron ddaF

(Lin et al., 2015)

axon | larval stage | progressive (with Df(3L)BSC773)

(Lin et al., 2015)

dendrite | larval stage (with Df(3L)BSC773)

(Lin et al., 2015)

larval brain | larval stage | nutrition conditional

(Feng et al., 2020)

larval multidendritic class IV neuron | cell autonomous | somatic clone

(Lin et al., 2015)

larval multidendritic class IV neuron | larval stage (with Df(3L)BSC773)

(Lin et al., 2015)

larval multidendritic class IV neuron | larval stage | progressive (with Df(3L)BSC773)

(Lin et al., 2015)

Detailed Description

Statement

Reference

path^dg50/path^dg50 mutants do not show significant defects in larval growth. path^dg50/Df(3L)BSC773 larvae show defects in C4da dendrite growth, with path^dg50/Df(3L)BSC773 mutants showing significant reductions in dendrite length compared to wild type. C4da dendrite growth is more severely affected in PNS neurons located in more posterior abdominal segments (A2 vs A5 vs A8), with significantly shorter dendrites and fewer branches in path^dg50/Df(3L)BSC773 larvae compared to wild type. Axonal arbors show progressive growth defects that are more severe in posterior segments (similar to controls prior to 48hr AEL, and by 120hr AEL C4da neurons in posterior segments show severe axon growth defects), compared to wild type. path^GFP/path^dg50 or path^GFP/path^Δ larvae show normal dendrite growth in C4da neurons.

(Lin et al., 2015)

path^dg50 larvae show severe dendrite growth defects in the large C4da and medium C3da dendritic arbors but no obvious effect on the smaller C1da arbor (or chordotonal or bipolar dendrite neurons) or whole animal growth. Early growth and patterning of C4da neurons proceed normally, but, beginning in late first larval instar larvae, two growth defects are manifest. First, C4da dendrite length plateaus at ~3.5 mm, which the mutants reach by ~36-40 h after egg laying, whereas control C4da dendrites continue growing throughout larval development, reaching ~20 mm. Second, C4da dendrite arbors are remodeled after reaching their maximum length in the mutants: terminal dendrites are progressively lost, whereas primary dendrites continue to grow, maintaining a constant value of total dendrite length.

path^KG06640/path^dg50 or path^dg50/Df(3L)BSC773 larvae show severe dendrite growth defects in the large C4da dendritic arbors.

C4da dendrites in larvae generated from path^dg50 germline clones arrested at the same value of dendrite growth as zygotic path^dg50 mutants.

path^dg50 C4da MARCM clones exhibit dendrite growth defects.

Cell size in the epidermis, oenocytes, fat body or salivary glands of path^dg50/Df(3L)BSC773 larvae is not reduced - only the size of neurons with extreme growth requirements (C4da/C3da neurons) appear to be affected.

(Lin et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

path^dg50/Df(3L)BSC773 has abnormal neuroanatomy | larval stage phenotype, non-enhanceable by Scer\GAL4^ppk.PG/pum^UAS.cYa

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has abnormal neuroanatomy | larval stage phenotype, non-enhanceable by Scer\GAL4^ppk.PG/RpL22^HMS00143

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has abnormal neuroanatomy | larval stage phenotype, non-enhanceable by Scer\GAL4^ppk.PG/S6k^KQ.UAS

(Lin et al., 2015)

Suppressed by

Statement

Reference

path^dg50/Df(3L)BSC773 has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^ppk.PG/pum^HMS01685

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by eIF4E1^UAS.cAa/Scer\GAL4^ppk.PG

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by Scer\GAL4^ppk.PG/S6k^TE.UAS

(Lin et al., 2015)

NOT suppressed by

Statement

Reference

path^dg50/Df(3L)BSC773 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Akt^UAS.Exel/Scer\GAL4^ppk.PG

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Rheb^UAS.cPa/Scer\GAL4^ppk.PG

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by raptor^UAS.Tag:HA/Scer\GAL4^ppk.PG

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by mTor^{WT.UAS.Tag:FLAG}/Scer\GAL4^ppk.PG

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Akt^{ΔPH.UAS.Tag:Myr(Unk)}/Scer\GAL4^ppk.PG

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Scer\GAL4^ppk.PG/Pi3K92E^CAAX.UAS

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Scer\GAL4^ppk.PG/Raf^UAS.cSa

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Scer\GAL4^ppk.PG/S6k^UAS.cMa

(Lin et al., 2015)

Suppressor of

Statement

Reference

path^dg50/Df(3L)BSC773 is a suppressor | partially of abnormal neuroanatomy phenotype of Scer\GAL4²²¹, ct^UAS.cPa

(Lin et al., 2015)

NOT Suppressor of

Statement

Reference

path^dg50/Df(3L)BSC773 is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Rac1^UAS.cLa, Scer\GAL4^ppk.PG

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 is a non-suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^98b, ct^UAS.cPa

(Lin et al., 2015)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

path^dg50/Df(3L)BSC773 has larval multidendritic class IV neuron phenotype, non-enhanceable by Scer\GAL4^ppk.PG/pum^UAS.cYa

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has larval multidendritic class IV neuron phenotype, non-enhanceable by Scer\GAL4^ppk.PG/RpL22^HMS00143

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has larval multidendritic class IV neuron phenotype, non-enhanceable by Scer\GAL4^ppk.PG/S6k^KQ.UAS

(Lin et al., 2015)

Suppressed by

Statement

Reference

path^dg50/Df(3L)BSC773 has larval multidendritic class IV neuron phenotype, suppressible | partially by Scer\GAL4^ppk.PG/pum^HMS01685

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has larval multidendritic class IV neuron phenotype, suppressible | partially by eIF4E1^UAS.cAa/Scer\GAL4^ppk.PG

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has larval multidendritic class IV neuron phenotype, suppressible | partially by Scer\GAL4^ppk.PG/S6k^TE.UAS

(Lin et al., 2015)

NOT suppressed by

Statement

Reference

path^dg50/Df(3L)BSC773 has larval multidendritic class IV neuron phenotype, non-suppressible by Scer\GAL4^ppk.PG/Pi3K92E^CAAX.UAS

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has larval multidendritic class IV neuron phenotype, non-suppressible by Scer\GAL4^ppk.PG/Raf^UAS.cSa

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has larval multidendritic class IV neuron phenotype, non-suppressible by Akt^{ΔPH.UAS.Tag:Myr(Unk)}/Scer\GAL4^ppk.PG

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has larval multidendritic class IV neuron phenotype, non-suppressible by Scer\GAL4^ppk.PG/S6k^UAS.cMa

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has larval multidendritic class IV neuron phenotype, non-suppressible by Akt^UAS.Exel/Scer\GAL4^ppk.PG

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has larval multidendritic class IV neuron phenotype, non-suppressible by Rheb^UAS.cPa/Scer\GAL4^ppk.PG

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has larval multidendritic class IV neuron phenotype, non-suppressible by raptor^UAS.Tag:HA/Scer\GAL4^ppk.PG

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 has larval multidendritic class IV neuron phenotype, non-suppressible by mTor^{WT.UAS.Tag:FLAG}/Scer\GAL4^ppk.PG

(Lin et al., 2015)

Suppressor of

Statement

Reference

path^dg50/Df(3L)BSC773 is a suppressor | partially of abdominal posterior ventral multidendritic neuron vdap phenotype of Scer\GAL4²²¹, ct^UAS.cPa

(Lin et al., 2015)

NOT Suppressor of

Statement

Reference

path^dg50/Df(3L)BSC773 is a non-suppressor of larval multidendritic class IV neuron phenotype of Rac1^UAS.cLa, Scer\GAL4^ppk.PG

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 is a non-suppressor of abdominal posterior ventral multidendritic neuron vdap phenotype of Scer\GAL4^98b, ct^UAS.cPa

(Lin et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Dendrite growth in path^dg50/Df(3L)BSC773 animals arrests at the same value in the absence or presence of Scer\GAL4^ppk.PG-mediated expression of Pi3K92E^{CAAX.Scer\UAS} or Raf^Scer\UAS.cSa.

(Lin et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

path^dg50 is rescued by Scer\GAL4^Act5C.PI/path^A.UAS.GFP

(Lin et al., 2015)

path^dg50 is rescued by path^A.UAS.GFP/Scer\GAL4^ppk.PG

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 is rescued by Scer\GAL4^elav-C155/path^A.UAS.GFP

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 is rescued by path^A.UAS.GFP/Scer\GAL4^ppk.PG

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 is rescued by Scer\GAL4^21-7/path^A.UAS.GFP

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 is rescued by path^E.UAS.GFP/Scer\GAL4^ppk.PG

(Lin et al., 2015)

Partially rescued by

path^dg50/Df(3L)BSC773 is partially rescued by path^A.UAS.GFP/Scer\GAL4^hs.PB

(Lin et al., 2015)

Not rescued by

path^dg50/Df(3L)BSC773 is not rescued by Scer\GAL4^Mhc.PK/path^A.UAS.GFP

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 is not rescued by path^A.UAS.GFP/Scer\GAL4^OK72

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 is not rescued by path^A.UAS.GFP/Scer\GAL4^A58

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 is not rescued by Scer\GAL4^ppk.PG/path^ΔN.UAS.GFP

(Lin et al., 2015)

path^dg50/Df(3L)BSC773 is not rescued by path^{ΔC.A.UAS.GFP}/Scer\GAL4^ppk.PG

(Lin et al., 2015)

Comments

Scer\GAL4^Act5C.PI-mediated expression of path^{A.Scer\UAS.T:Avic\GFP} rescues the dendrite defects in path^dg50 mutants.

A heat-shock induced pulse of expression of path^{A.Scer\UAS.T:Avic\GFP} (using Scer\GAL4^hs.PB) at 24, 48, 72 or 96 hours AEL is sufficient to significantly rescue dendrite growth defects of path^dg50/Df(3L)BSC773 animals.

path^{A.Scer\UAS.T:Avic\GFP} expression in path^dg50 MARCM clones rescues their C4da dendrite growth defects.

Scer\GAL4^ppk.PG-mediated expression of path^{A.Scer\UAS.T:Avic\GFP} or path^{A.Scer\UAS.T:Avic\GFP}, but not path^{ΔN.Scer\UAS.T:Avic\GFP} or path^{ΔC.A.Scer\UAS.T:Avic\GFP}, rescues the dendrite growth defects of path^dg50/Df(3L)BSC773 animals.

(Lin et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dg50

(Lin et al., 2015)

path^dg50

(Tenedini et al., 2025, Feng et al., 2020, Lin et al., 2015, Lin et al., 2015)

Name Synonyms

dendrite growth defective 50

(Lin et al., 2015)

Secondary FlyBase IDs

References (5)

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