FlyBase Allele Report: Dmel\Nlg3[KO127]

General Information

Symbol

Dmel\Nlg3^KO127

Species

D. melanogaster

Name

FlyBase ID

FBal0301059

Feature type

allele

Associated gene

Dmel\Nlg3

Associated Insertion(s)

TI{TI}Nlg3^KO127

Carried in Construct

Also Known As

dnlg3^KO127

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Xing et al., 2014)

Mutagen

ends-out gene targeting

(Xing et al., 2014)

Progenitor genotype

Associated Insertion(s)

TI{TI}Nlg3^KO127

Cytology

Description

3849bp of the Nlg3 genomic region (nucleotides -1556 to +2293) have been replaced by a loxP cassette containing a w^+mW.hs marker.

(Xing et al., 2014)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3R:7,601,456..7,605,303 [-]

Comment:

Nlg3 genomic region (nucleotides -1556 to +2293, boundaries inferred from primer sequences) have been replaced by a P1\loxP cassette containing a w[+mW.hs] marker.

(Xing et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of autism spectrum disorder

CEA with Nrx-1^Δ83

(Guangming et al., 2023)

CEA with Nlg2^KO70

(Guangming et al., 2023)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Nlg3^KO127/Nlg3^KO127 and Nlg3^KO127/Nlg3^KO88 and Nlg3^KO127 hemizygous mutant adults show decreased daily locomotor activity, as compared to controls.

The expression of either Nlg3^Δ10.UAS or Nlg3^UAS.cXa under the control of Scer\GAL4^how-24B rescues the increased bouton number in the third instar larval neuromuscular junction of Nlg3^KO127, even leading to fewer boutons compared to controls.

(Wu et al., 2018)

Nlg3^KO127 homozygous mutant adults do not show any defects in the medulla columnar restriction of the axonal branches of L4 lamina neurons.

(Liu et al., 2017)

Excitatory junctional potential (EJP) amplitude and miniature excitatory junctional potential (mEJP) frequency are significantly reduced at the mutant third larval instar neuromuscular junction compared to wild type. mEJP amplitude is not significantly affected.

Nlg3^KO127/Nlg3^f00777 third larval instar larvae show a significant increase in bouton number and a significant decrease in bouton size at the neuromuscular junction compared to wild type. Synaptic branch number at the neuromuscular junction is not significantly affected.

(Xing et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Nlg3^KO127 is rescued by Nlg3^UAS.cXa/Scer\GAL4^elav.PU

(Wu et al., 2018)

Nlg3^KO127 is rescued by Scer\GAL4^elav.PU/Nlg3^s.UAS

(Wu et al., 2018)

Nlg3^KO88/Nlg3^KO127 is rescued by Nlg3^UAS.cXa/Scer\GAL4^Mhc.PU

(Xing et al., 2014)

Partially rescued by

Nlg3^KO127 is partially rescued by Scer\GAL4^elav.PU/Nlg3^Δ10.UAS

(Wu et al., 2018)

Not rescued by

Nlg3^KO127 is not rescued by Scer\GAL4^how-24B/Nlg3^Δ10.UAS

(Wu et al., 2018)

Nlg3^KO127 is not rescued by Scer\GAL4^elav.PU/Nlg3^Δ10.UAS

(Wu et al., 2018)

Nlg3^KO127 is not rescued by Scer\GAL4^elav.PU/Nlg3^{NTF.UAS.Tag:HA}

(Wu et al., 2018)

Nlg3^KO127 is not rescued by Scer\GAL4^how-24B/Nlg3^{NTF.UAS.Tag:HA}

(Wu et al., 2018)

Nlg3^KO127 is not rescued by Nlg3^UAS.cXa/Scer\GAL4^how-24B

(Wu et al., 2018)

Nlg3^KO127 is not rescued by Scer\GAL4^how-24B/Nlg3^s.UAS

(Wu et al., 2018)

Comments

The expression of Nlg3^Δ10.UAS under the control of Scer\GAL4^how-24B does not rescue the adult locomotor activity of Nlg3^KO127 mutants; it also rescues the increased bouton number in the third instar larval neuromuscular junction, even leading to fewer boutons compared to controls.

The expression of Nlg3^UAS.cXa under the control of Scer\GAL4^how-24B rescues the increased bouton number in the third instar larval neuromuscular junction of Nlg3^KO127 mutants, even leading to fewer boutons compared to controls.

(Wu et al., 2018)

Muscle-specific expression of Nlg3^Scer\UAS.cXa (under the control of Scer\GAL4^Mhc.PU) rescues the defects in bouton number and size seen at the neuromuscular junction in Nlg3^KO88/Nlg3^KO127 larvae. Neuron-specific expression (under the control of Scer\GAL4^elav.PU) does not rescue this phenotype.

(Xing et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Nlg3^KO127

dnlg3^KO127

(Guangming et al., 2023, Wu et al., 2018, Liu et al., 2017, Xing et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close