FlyBase Allele Report: Dmel\SCAR[NIG.4636R]

General Information

Symbol

Dmel\SCAR^NIG.4636R

Species

D. melanogaster

Name

FlyBase ID

FBal0269212

Feature type

allele

Associated gene

Dmel\SCAR

Associated Insertion(s)

Carried in Construct

P{NIG.4636R}

Also Known As

wave RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Stephan et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Stephan et al., 2011)

Mutagen

in vitro construct

(Stephan et al., 2011)

Progenitor genotype

Carried in construct

P{NIG.4636R}

Cytology

Description

UASt regulatory sequences drive expression of an RNAi construct targeting SCAR.

(Stephan et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-4636R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration, with Scer\GAL4^Hml.Δ

(Sander et al., 2013)

abnormal cell migration | oogenesis, with Scer\GAL4^c306

(Rötte et al., 2024)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav-C155

(Stephan et al., 2011)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^sca.PU

(Stephan et al., 2011)

abnormal wound healing | P-stage, with Scer\GAL4^A58

(Rötte et al., 2024)

Phenotype Manifest In

actin cytoskeleton, with Scer\GAL4^Hml.Δ

(Sander et al., 2013)

border follicle cell, with Scer\GAL4^c306

(Rötte et al., 2024)

embryonic/larval plasmatocyte, with Scer\GAL4^Hml.Δ

(Chen et al., 2014)

lamellipodium, with Scer\GAL4^Hml.Δ

(Chen et al., 2014)

lamellipodium | P-stage, with Scer\GAL4^A58

(Rötte et al., 2024)

tract | third instar larval stage

(Stephan et al., 2011)

Detailed Description

Statement

Reference

Macrophage-specific expression of SCAR^NIG.4636R using the Scer\GAL4^Hml.Δ driver completely disrupts lamellipodia formation.

(Chen et al., 2014)

Expression of SCAR^NIG.4636R in macrophages from larval stage onwards (under the control of Scer\GAL4^Hml.Δ) results in an overall reduction of the actin meshwork and exhibit prominent filopodia consisting of densely packed bundles of actin filaments.

Laser-induced single cell ablation or wounding of the wing epithelial cells in wild-type flies results in cells migrating over long distances to the wound site, while in flies expressing SCAR^NIG.4636R under the control of Scer\GAL4^Hml.Δ the cells fail to migrate towards the wound. Knockdown cells send long filopodial protrusions toward the wound, indicating that the chemotactic response is not affected.

(Sander et al., 2013)

Eye disc specific expression of SCAR^NIG.4636R under the control of Scer\GAL4^GMR.PU does not affect axonal targeting in the visual system.

Third instar larvae expressingSCAR^NIG.4636R under the control of the pan-neuronal drivers Scer\GAL4^elav-C155 or Scer\GAL4^sca.PU display axonal targeting defects.

(Stephan et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

SCAR^NIG.4636R, Scer\GAL4^A58 has abnormal wound healing | P-stage phenotype, non-enhanceable by Scer\GAL4^unspecified/cyri^KK108589

(Rötte et al., 2024)

Suppressed by