FlyBase Allele Report: Dmel\Smox[RNAi.UAS.cPa]

General Information

Symbol

Dmel\Smox^RNAi.UAS.cPa

Species

D. melanogaster

Name

FlyBase ID

FBal0268514

Feature type

allele

Associated gene

Dmel\Smox

Associated Insertion(s)

Carried in Construct

P{UAS-Smox.RNAi.P}

Also Known As

idSmad2

Key Links

Transgenic product class

RNAi_reagent

(Peterson et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Peterson et al., 2012)

Mutagen

in vitro construct

(Peterson et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Smox.RNAi.P}

Cytology

Description

UAS regulatory sequences drive expression of an RNAi construct targeting nt 1469-1928 of the Smox transcript.

(Peterson et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Smox

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Ubiquitous expression of Smox^{dsRNA.Scer\UAS.cPa} under the control of Scer\GAL4^da.G32 results in widening of the larval wing disc. Widening is also seen with the pan-wing disc drivers Scer\GAL4^sd-SG29.1 or Scer\GAL4^32B, No widening is seen when Smox^{dsRNA.Scer\UAS.cPa} is expressed under the control of either of the partial wing disc drivers Scer\GAL4^nub-AC-62 or Scer\GAL4^tsh-md621, but widening is detected when both drivers are used simultaneously. No widening is seen with the partial wing disc drivers Scer\GAL4^A9 or Scer\GAL4^71B and minimal widening is seen with Scer\GAL4^C-765. Wings discs expressing Smox^{dsRNA.Scer\UAS.cPa} with either Scer\GAL4^C-765 or Scer\GAL4^nub-AC-62 and the prothoracic gland driver Scer\GAL4^phm.PO also appear normal. Expression of Smox^{dsRNA.Scer\UAS.cPa} under the control of Scer\GAL4^esg-05730B also produces the wing disc widening phenotype.

(Peterson and O'Connor, 2013)

Expression of Smox^{dsRNA.Scer\UAS.cPa} under the control of Scer\GAL4^vg.MQ.Exel results in moderately blistered wings.

(Peterson et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^NP5130, Smox^RNAi.UAS.cPa has increased size | larval stage phenotype, suppressible by babo^RNAi.a.UAS/Scer\GAL4^NP5130

(Upadhyay et al., 2020)

Scer\GAL4^esg-05730B, Smox^RNAi.UAS.cPa has abnormal size | third instar larval stage phenotype, suppressible by Scer\GAL4^esg-05730B/babo^RNAi.UAS

(Peterson and O'Connor, 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^NP5130, Smox^RNAi.UAS.cPa has increased size | larval stage phenotype, non-suppressible by babo^RNAi.b.UAS/Scer\GAL4^NP5130

(Upadhyay et al., 2020)

Scer\GAL4^NP5130, Smox^RNAi.UAS.cPa has increased size | larval stage phenotype, non-suppressible by babo^RNAi.c.UAS/Scer\GAL4^NP5130

(Upadhyay et al., 2020)

Enhancer of

Statement

Reference

Scer\GAL4^vg.MQ.Exel/Smox^RNAi.UAS.cPa is an enhancer of visible phenotype of Scer\GAL4^vg.MQ.Exel, babo^Q302D.UAS

(Peterson et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^NP5130, Smox^RNAi.UAS.cPa has wing pouch | larval stage phenotype, suppressible by babo^RNAi.a.UAS/Scer\GAL4^NP5130

(Upadhyay et al., 2020)

Scer\GAL4^esg-05730B, Smox^RNAi.UAS.cPa has wing disc | third instar larval stage phenotype, suppressible by Scer\GAL4^esg-05730B/babo^RNAi.UAS

(Peterson and O'Connor, 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^NP5130, Smox^RNAi.UAS.cPa has wing pouch | larval stage phenotype, non-suppressible by babo^RNAi.b.UAS/Scer\GAL4^NP5130

(Upadhyay et al., 2020)

Scer\GAL4^NP5130, Smox^RNAi.UAS.cPa has wing pouch | larval stage phenotype, non-suppressible by babo^RNAi.c.UAS/Scer\GAL4^NP5130

(Upadhyay et al., 2020)

Enhancer of

Statement

Reference

Scer\GAL4^vg.MQ.Exel/Smox^RNAi.UAS.cPa is an enhancer of wing blade phenotype of Scer\GAL4^vg.MQ.Exel, babo^Q302D.UAS

(Peterson et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of babo^{dsRNA.Scer\UAS} suppresses the wing discs widening phenotype seen in third instar larvae expressing Smox^{dsRNA.Scer\UAS.cPa} under the control of Scer\GAL4^esg-05730B.

(Peterson and O'Connor, 2013)

Co-expression of Smox^{dsRNA.Scer\UAS.cPa} with babo^{Q302D.Scer\UAS} under the control of Scer\GAL4^vg.MQ.Exel makes the babo^{Q302D.Scer\UAS}-induced phenotype more severe. The resulting wings are more wrinkled and shrunken than wings where either construct is expressed alone.

(Peterson et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Smox^RNAi.UAS.cPa

Smox^{dsRNA.Scer\UAS.cPa}

Smox^{dsRNA.UAS.cPa}

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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