FlyBase Allele Report: Dmel\rl[GD4697]

General Information

Symbol

Dmel\rl^GD4697

Species

D. melanogaster

Name

FlyBase ID

FBal0241100

Feature type

allele

Associated gene

Dmel\rl

Associated Insertion(s)

Carried in Construct

P{GD4697}

Also Known As

UAS-rolled-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Franzdóttir et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Franzdóttir et al., 2009)

Mutagen

in vitro construct

(Franzdóttir et al., 2009)

Progenitor genotype

Carried in construct

P{GD4697}

Cytology

Description

UASt regulatory sequences drive expression of a rl RNAi construct.

(Franzdóttir et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4697

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates carcinoma

modeled by Ras85D^V12.UAS

(Murcia et al., 2019)

modeled by Ras85D^V12.UAS, scrib^KK101128

(Murcia et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^phtm.PO

(Rewitz et al., 2009)

abnormal size | adult stage, with Scer\GAL4^elav.PU

(Murcia et al., 2019)

increased body size, with Scer\GAL4^phtm.PO

(Rewitz et al., 2009)

increased cell death | third instar larval stage, with Scer\GAL4^hh.PU

(Murcia et al., 2019)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible | adult stage, with Scer\GAL4^elav.PU

(Murcia et al., 2019)

Phenotype Manifest In

eye, with Scer\GAL4^elav.PU

(Murcia et al., 2019)

glial cell, with Scer\GAL4^repo

(Franzdóttir et al., 2009)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^en-e16E

(Hahn et al., 2013)

wing disc | third instar larval stage, with Scer\GAL4^hh.PU

(Murcia et al., 2019)

wing vein, with Scer\GAL4^en-e16E

(Hahn et al., 2013)

Detailed Description

Statement

Reference

The expression of rl^GD4697 for 3 days during larval development under the combined control of Scer\GAL4^hh.PU and Gal80[ts] leads to third instar larval wing discs showing an increase in apoptosis (Dcp1 immunostaining), particularly upon irradiation.

(Murcia et al., 2019)

Expression of rl^GD4697 in the posterior wing compartment, under the control of Scer\GAL4^en-e16E, generates a wing vein differentiation phenotype.

(Hahn et al., 2013)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or late pupal lethality.

(Schnorrer et al., 2010)

Expression of rl^GD4697 under the control of Scer\GAL4^repo results in lack of glial differentiation in the eye disc, but only mildly affects glial division or migration.

(Franzdóttir et al., 2009)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 10-20% or 40-50% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 20-30% or 90-100% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

Scer\GAL4^phm.PO-driven overexpression of rl^GD4697 delays pupariation by 6.1 days. The developmental delay, as well as the size increase caused by Scer\GAL4^phm.PO-driven overexpression of Ras85D^GD12553 is negated by 20-hydroxyecdysone feeding

(Rewitz et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4697

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^hh.PU, rl^GD4697 has increased cell death | third instar larval stage phenotype, enhanceable by Ras85D^V12.UAS, Scer\GAL4^hh.PU

(Murcia et al., 2019)

Scer\GAL4^hh.PU, rl^GD4697 has increased cell death | third instar larval stage phenotype, enhanceable by Ras85D^V12.UAS/scrib^KK101128, Scer\GAL4^hh.PU

(Murcia et al., 2019)

Suppressed by

Statement

Reference

Scer\GAL4^hh.PU, rl^GD4697 has increased cell death | third instar larval stage phenotype, suppressible by Scer\GAL4^hh.PU/p53^GD4483

(Murcia et al., 2019)

Scer\GAL4^hh.PU, rl^GD4697 has increased cell death | third instar larval stage phenotype, suppressible by Scer\GAL4^hh.PU/rpr^RNAi.RHG.UAS/grim^RNAi.RHG.UAS/hid^RNAi.RHG.UAS

(Murcia et al., 2019)

Scer\GAL4^elav.PU, rl^GD4697 has abnormal size | adult stage phenotype, suppressible by Scer\GAL4^elav.PU/p53^GD4483

(Murcia et al., 2019)

Scer\GAL4^elav.PU, rl^GD4697 has visible | adult stage phenotype, suppressible by Scer\GAL4^elav.PU/p53^GD4483

(Murcia et al., 2019)

Ras85D^V12.UAS, Scer\GAL4^hh.PU, rl^GD4697 has increased cell death | third instar larval stage phenotype, suppressible | partially by Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^hh.PU

(Murcia et al., 2019)

Scer\GAL4^hh.PU, rl^GD4697 has increased cell death | third instar larval stage phenotype, suppressible | conditional by Ras85D^V12.UAS/scrib^KK101128, Scer\GAL4^hh.PU

(Murcia et al., 2019)

Suppressor of

Statement

Reference

rl^GD4697, Scer\GAL4^hh.PU is a suppressor of decreased cell death | third instar larval stage | conditional phenotype of Ras85D^V12.UAS, Scer\GAL4^hh.PU

(Murcia et al., 2019)

NOT Suppressor of

Statement

Reference

p53^GD4483/rl^GD4697, Scer\GAL4^hh.PU is a non-suppressor of decreased cell death | third instar larval stage | conditional phenotype of Ras85D^V12.UAS, Scer\GAL4^hh.PU

(Murcia et al., 2019)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^hh.PU, rl^GD4697 has wing disc | third instar larval stage phenotype, enhanceable by Ras85D^V12.UAS, Scer\GAL4^hh.PU

(Murcia et al., 2019)

Scer\GAL4^hh.PU, rl^GD4697 has wing disc | third instar larval stage phenotype, enhanceable by Ras85D^V12.UAS/scrib^KK101128, Scer\GAL4^hh.PU

(Murcia et al., 2019)

Scer\GAL4^en-e16E, rl^GD4697 has wing phenotype, enhanceable by Scer\GAL4^en-e16E/step^RNAi.UAS

(Hahn et al., 2013)

Scer\GAL4^en-e16E, rl^GD4697 has wing vein phenotype, enhanceable by Scer\GAL4^en-e16E/step^RNAi.UAS

(Hahn et al., 2013)

NOT Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, rl^GD4697 has wing phenotype, non-enhanceable by Scer\GAL4^en-e16E/step^UAS.FL

(Hahn et al., 2013)

Scer\GAL4^en-e16E, rl^GD4697 has wing vein phenotype, non-enhanceable by Scer\GAL4^en-e16E/step^UAS.FL

(Hahn et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^hh.PU, rl^GD4697 has wing disc | third instar larval stage phenotype, suppressible | conditional by Ras85D^V12.UAS/scrib^KK101128, Scer\GAL4^hh.PU

(Murcia et al., 2019)

Scer\GAL4^hh.PU, rl^GD4697 has wing disc | third instar larval stage phenotype, suppressible by Scer\GAL4^hh.PU/p53^GD4483

(Murcia et al., 2019)

Scer\GAL4^hh.PU, rl^GD4697 has wing disc | third instar larval stage phenotype, suppressible by Scer\GAL4^hh.PU/rpr^RNAi.RHG.UAS/grim^RNAi.RHG.UAS/hid^RNAi.RHG.UAS

(Murcia et al., 2019)

Scer\GAL4^elav.PU, rl^GD4697 has eye phenotype, suppressible by Scer\GAL4^elav.PU/p53^GD4483

(Murcia et al., 2019)

Ras85D^V12.UAS, Scer\GAL4^hh.PU, rl^GD4697 has wing disc | third instar larval stage phenotype, suppressible | partially by Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^hh.PU

(Murcia et al., 2019)

NOT suppressed by

Statement

Reference

Scer\GAL4^en-e16E, rl^GD4697 has wing phenotype, non-suppressible by Scer\GAL4^en-e16E/step^UAS.FL

(Hahn et al., 2013)

Scer\GAL4^en-e16E, rl^GD4697 has wing vein phenotype, non-suppressible by Scer\GAL4^en-e16E/step^UAS.FL

(Hahn et al., 2013)

Suppressor of

Statement

Reference

rl^GD4697, Scer\GAL4^hh.PU is a suppressor of wing disc | third instar larval stage | conditional phenotype of Ras85D^V12.UAS, Scer\GAL4^hh.PU

(Murcia et al., 2019)

NOT Suppressor of

Statement

Reference

p53^GD4483/rl^GD4697, Scer\GAL4^hh.PU is a non-suppressor of wing disc | third instar larval stage | conditional phenotype of Ras85D^V12.UAS, Scer\GAL4^hh.PU

(Murcia et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of rl^GD4697 reduces hemocyte proliferation induced by expression of Ras85D^V12.Scer\UAS via Scer\GAL4^Hml.PG.

(Ashton-Beaucage et al., 2014)

Knockdown of rl in a step^{dsRNA.Scer\UAS} background, through expression of rl^GD4697 in the posterior wing compartment (both transgenes under the control of Scer\GAL4^en-e16E) generates a more severe rl phenotype than in a wild-type background. The lateral vein L4 is entirely lost, both cross veins are not differentiated and the distal end of the lateral vein L5 is missing.

Expression of step^Scer\UAS.FL has no effect on the wing vein defects caused by expression of rl^GD4697 in the posterior compartment of the wing, under the control of Scer\GAL4^en-e16E.

(Hahn et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v43123