FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Rab7T22N.UASp.YFP
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General Information
Symbol
Dmel\Rab7T22N.UASp.YFP
Species
D. melanogaster
Name
FlyBase ID
FBal0215399
Feature type
allele
Associated gene
Dmel\Rab7
Associated Insertion(s)
Carried in Construct
P{UASp-YFP.Rab7.T22N}
Also Known As
UAS-Rab7DN, rab7DN, Rab7-DN, UASp-YFP.Rab7.T22N, UAS-YFP-Rab7DN, Rab7 DN, UAS-Rab7T22N, UASp-YFP-Rab7T22N, UAS-YFP.Rab7.T22N, UAS-Rab7DN
Key Links
Transgenic product class
dominant_negative_variant
(Zhang et al., 2007)
missense_variant
(Zhang et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Zhang et al., 2007)
missense_variant
(Zhang et al., 2007)
Mutagen
in vitro construct
(Zhang et al., 2007, Zhang et al., 2007)
Progenitor genotype
Carried in construct
P{UASp-YFP.Rab7.T22N}
Cytology
Description

Amino acid replacement: T22N.

(Zhang et al., 2007)

UASp regulatory sequences drive expression of a predicted dominant negative form of Rab7 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Rab7
Tagged with
YFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  tauopathy
      modeled by Hsap\MAPTGMR.Ex.PJ
      (Bakhoum et al., 2014)
      ameliorates  motor neuron disease
      modeled by scatHMS01910
      (Patel et al., 2020)
      exacerbates  tauopathy
      modeled by Hsap\MAPTUAS.2N4R.cSa
      (Asadzadeh et al., 2022)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos from mothers expressing Rab7T22N.UASp.YFP under the control of Scer\GAL4VP16.mat.αTub67C show larger yolk granules.

      (Lattao et al., 2021)

      Scer\GAL4He.PZ-driven expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP does not induce increase in the number of circulating hemocytes in third instar larvae relative to controls.

      (Del Signore et al., 2017)

      Animals expressing Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4grh.D4 are viable, no obvious defects in larval dorsal trunk elongation and airway morphology are observed and the larvae are of comparable size to age-matched controls.

      (Wingen et al., 2017)

      Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4btl.PU in embryos does not cause significant defects in dorsal branch fusion.

      (Caviglia et al., 2016)

      Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 results in formation of abnormal large vesicular structures in the egg chambers.

      (Liu et al., 2015)

      Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP in secondary cells under the control of Scer\GAL4dsx.KI reduces exosome secretion into the accessory gland lumen compared to controls.

      (Corrigan et al., 2014)

      Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4CY2 does not change the average lysosome size as compared to wild-type.

      (Dodson et al., 2014)

      Adult hemocytes expressing Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4Hml.Δ show slower maturation of phagosomes containing pHrodo-conjugated S. aureus compared to wild-type controls.

      Adults expressing Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4Hml.Δ show significantly decreased survival compared to controls after injection with S. aureus.

      (Garg and Wu, 2014)

      Embryos derived from females expressing Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 show a significant delay in the clearance of the paternal mitochondrial derivatives compared to wild type.

      (Politi et al., 2014)

      The fraction of oocytes showing endocytic activity is reduced compared to wild type in females expressing Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16.

      (Tsai et al., 2014)

      Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4eg-Mz360 in commissural axons in the embryo does not disrupt their outgrowth across the midline.

      Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of either Scer\GAL4eve.RN2 or Scer\GAL4elav-C155 does not result in any defects in axon outgrowth in the embryo.

      (van den Brink et al., 2013)

      Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4slbo.2.6 impairs border cell migration in the developing egg chamber. No border cell cluster dissociation is seen.

      (Chen et al., 2012)

      Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4CY2 results in a 46% decrease in lysosome clustering in the follicle cells compared to controls.

      (Dodson et al., 2012)

      Expression via Scer\GAL4pnr-MD237 results in flies with a wild type notum.

      (Thomas et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhancer of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Suppressed by
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increased number of circulating hemocytes along with the increase in F-actin and the expansion of the LysoTracker-positive compartments in the mutant cells characteristic for OcrlΔ3 mutant third instar larvae is partially suppressed by Scer\GAL4He.PZ-driven expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP, but the adult lethality of OcrlΔ3 animals is not rescued.

      (Del Signore et al., 2017)

      The lysosome expansion phenotype observed in third instar larvae expressing FIG4dsRNA.miRNA.Scer\UAS under the control of Scer\GAL4Mef2.PU is suppressed by co-expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP.

      (Bharadwaj et al., 2016)

      Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4CY2 does not change the average lysosome size in Lrrk mutants.

      (Dodson et al., 2014)

      The reduction in the fraction of oocytes showing endocytic activity caused by expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 is suppressed by co-expression of EndoBScer\UAS.P\T.T:Zzzz\FLAG.

      (Tsai et al., 2014)

      The increased clustering of lysosomes which is seen in the follicle cells of females expressing LrrkG1914S.Scer\UAS under the control of Scer\GAL4CY2 is significantly suppressed by co-expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP.

      (Dodson et al., 2012)

      Co-expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP weakly suppresses the uninflated wing phenotype of Scer\GAL4Ccap.PP, OsbpScer\UAS.cMa flies.

      (Ma et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference

      The formation of giant autophagic bodies in the retina of adult flies expressing Hsap\MAPTGMR.Ex.PJ is partially suppressed by co-expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4GMR.PU.

      (Bakhoum et al., 2014)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (7)
      Reported As
      Symbol Synonym
      Rab7DN
      (Lambert et al., 2022, Del Signore et al., 2017, Kamalesh et al., 2017)
      Rab7SN
      (Del Signore et al., 2017)
      Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP
      Rab7T22N.UASp.YFP
      Rab7T22N
      (Hertenstein et al., 2021)
      rab7DN
      (Dodson et al., 2012)
      rab7T22N
      (Liu et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (48)