FlyBase Allele Report: Dmel\Imp[GD9232]

General Information

Symbol

Dmel\Imp^GD9232

Species

D. melanogaster

Name

FlyBase ID

FBal0207375

Feature type

allele

Associated gene

Dmel\Imp

Associated Insertion(s)

Carried in Construct

P{GD9232}

Also Known As

UAS-imp-RNAi, UAS-imp^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD9232}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9232

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of acquired metabolic disease

CEA

(Kawasaki et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates brain cancer

modeled by pros^KK109284

(Narbonne-Reveau et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ppk.PG

(Hattori et al., 2013)

abnormal starvation stress response, with Scer\GAL4^elav.PU

(Kawasaki et al., 2015)

long lived, with Scer\GAL4^elav.PU

(Kawasaki et al., 2015)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

abdominal dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4^ppk.PG

(Hattori et al., 2013)

dendritic tree | third instar larval stage, with Scer\GAL4^ppk.PG

(Hattori et al., 2013)

Detailed Description

Statement

Reference

Expression of Imp^GD9232 under the control of Scer\GAL4^esg.PU, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, does not cause any obvious midgut phenotypes, compared to controls.

(Mundorf et al., 2019)

Flies expressing Imp^GD9232 under the control of Scer\GAL4^elav.PU have a normal hatching rate and developmental growth. No morphological defects are observed. However, hemolymph trehalose levels are significantly higher than in controls in both a fed and starved state. Normalised whole body trehalose is only increased in fasting conditions. Hemolymph protein concentration is unchanged in both the fed and starved condition.

Flies expressing Imp^GD9232 under the control of Scer\GAL4^elav.PU consistently show an increase in average and maximum lifespan over that of control flies. Lifespan is reduced compared to controls following starvation. Body size and weight are unchanged.

(Kawasaki et al., 2015)

The expression of Imp^GD9232 under the control of Scer\GAL4²²¹, in combination with Dicer-2 for efficient RNAi, results in third instar larval class I abdominal dorsal multidendritic ddaD and ddaE neurons exhibiting no significant difference in the numbers of dendrite branch terminals, as compared to controls.

The expression of Imp^GD9232 under the control of Scer\GAL4^ppk.PG, in combination with Dicer-2 for efficient RNAi, results in third instar larval class IV abdominal dorsal multidendritic ddaC neurons exhibiting dendrite branch terminals of similar number, but of significantly lower average length, as compared to controls.

(Hattori et al., 2013)

Adults expressing Imp^GD9232 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9232

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Imp^GD9232, Scer\GAL4^esg.PU is a suppressor of increased occurrence of cell division | adult stage phenotype of Ets21C^{UAS.LONG.Tag:MYC}, Scer\GAL4^esg.PU

(Mundorf et al., 2019)

Imp^GD9232, Scer\GAL4²²¹ is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA}

(Hattori et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

Imp^GD9232, Scer\GAL4^esg.PU is a suppressor of intestinal stem cell phenotype of Ets21C^{UAS.LONG.Tag:MYC}, Scer\GAL4^esg.PU

(Mundorf et al., 2019)

Imp^GD9232, Scer\GAL4^esg.PU is a suppressor | partially of adult midgut epithelium phenotype of Ets21C^{UAS.LONG.Tag:MYC}, Scer\GAL4^esg.PU

(Mundorf et al., 2019)

Imp^GD9232, Scer\GAL4²²¹ is a suppressor of abdominal dorsal multidendritic neuron ddaD | third instar larval stage phenotype of Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA}

(Hattori et al., 2013)

Imp^GD9232, Scer\GAL4²²¹ is a suppressor of abdominal dorsal multidendritic neuron ddaE | third instar larval stage phenotype of Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA}

(Hattori et al., 2013)

Imp^GD9232, Scer\GAL4²²¹ is a suppressor of dendritic tree | third instar larval stage phenotype of Scer\GAL4²²¹, kn^{UAS.cHa.Tag:HA}

(Hattori et al., 2013)

NOT Suppressor of

Statement

Reference

Imp^GD9232, Scer\GAL4^NP0001 is a non-suppressor of adult midgut epithelium phenotype of Ets21C^{UAS.LONG.Tag:MYC}, Scer\GAL4^NP0001

(Mundorf et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of dendrite terminals in third instar larval class I abdominal dorsal multidendritic ddaD and ddaE neurons expressing kn^{Scer\UAS.cHa.T:Ivir\HA1} under the control of Scer\GAL4²²¹ is partially suppressed by co-expressing Imp^GD9232.

(Hattori et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v20321