FlyBase Allele Report: Dmel\wg[UAS.GFP]

General Information

Symbol

Dmel\wg^UAS.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0138114

Feature type

allele

Associated gene

Dmel\wg

Associated Insertion(s)

Carried in Construct

P{UAS-GFP-wg}

Also Known As

UAS-GFP-wg, UAS-GFP-wingless, UAS-wg::GFP

Key Links

Transgenic product class

wild_type

(Pfeiffer et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Pfeiffer et al., 2002)

Mutagen

in vitro construct

(Pfeiffer et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-GFP-wg}

Cytology

Description

UAS sequences drive expression of wg tagged with GFP, which has had been inserted in between Trp 38 and 39. The 3' UTR of wg is missing.

(Pfeiffer et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of P-stage | heat sensitive, with Scer\GAL4^bbg-C96

(Vuong et al., 2014)

partially lethal - majority live, with Scer\GAL4^wg-Gal4, wg^Gal4/wg^l-17

(Won et al., 2019)

Phenotype Manifest In

wing | heat sensitive, with Scer\GAL4^bbg-C96

(Vuong et al., 2014)

wing margin bristle | increased number, with Scer\GAL4^nub.PU

(Won et al., 2019)

Detailed Description

Statement

Reference

Transient expression of wg^UAS.GFP under the combined control of Scer\GAL4^nub.PU and Gal80[ts] induces ectopic bristles in the wing margin.

(Won et al., 2019)

Transient expression of wg^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^nub.PU (using UAS-Gal80[ts] to limit the time of expression to late third larval instar/early pupa stage) has no effect on adult wing morphology or lethality.

(Kim et al., 2016)

Expression of wg^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^bbg-C96 has little effect on the wing at 22[o]C, but expression at 25[o]C leads to 100% early pupal lethality without detectable wing development.

(Vuong et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^bbg-C96, wg^UAS.GFP has lethal - all die before end of P-stage | heat sensitive phenotype, suppressible | partially by Klp64D^NIG.10642R, Scer\GAL4^bbg-C96

(Vuong et al., 2014)

NOT Suppressor of

Statement

Reference

wg^UAS.GFP, Scer\GAL4^bbg-C96 is a non-suppressor of visible phenotype of Klp64D^NIG.10642R, Scer\GAL4^bbg-C96

(Vuong et al., 2014)

Other

Statement

Reference

Scer\GAL4^nub.PU, sona^UAS.cKa, wg^UAS.GFP has visible phenotype

(Kim et al., 2016)

Klp64D^NIG.10642R, Scer\GAL4^bbg-C96, wg^UAS.GFP has visible phenotype

(Vuong et al., 2014)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

wg^UAS.GFP, Scer\GAL4^bbg-C96 is a non-suppressor of wing margin phenotype of Klp64D^NIG.10642R, Scer\GAL4^bbg-C96

(Vuong et al., 2014)

Other

Statement

Reference

Scer\GAL4^nub.PU, sona^UAS.cKa, wg^UAS.GFP has wing margin bristle | ectopic phenotype

(Kim et al., 2016)

Scer\GAL4^nub.PU, sona^UAS.cKa, wg^UAS.GFP has wing blade phenotype

(Kim et al., 2016)

Klp64D^NIG.10642R, Scer\GAL4^bbg-C96, wg^UAS.GFP has wing phenotype

(Vuong et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of both wg^{Scer\UAS.T:Avic\GFP} and sona^Scer\UAS.cKa simultaneously under the control of Scer\GAL4^nub.PU (using UAS-Gal80[ts] to limit the time of expression to late third larval instar/early pupa stage) causes high degree of lethality and the surviving adults display wing phenotypes that include multiple ectopic bristles near the anterior wing margin neither of which is observed when wg^{Scer\UAS.T:Avic\GFP} or sona^Scer\UAS.cKa is expressed alone.

(Kim et al., 2016)

Expression of wg^{Scer\UAS.T:Avic\GFP} does not suppress the wing notching seen when Klp64D^NIG.10642R is expressed under the control of Scer\GAL4^bbg-C96.

Expression of Klp64D^NIG.10642R partially rescues the lethality seen when wg^{Scer\UAS.T:Avic\GFP} is expressed under the control of Scer\GAL4^bbg-C96 at 25[o]C, resulting in the survival of 30% of adult flies with small wings.

(Vuong et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^en-e16E/wg^UAS.GFP rescues wg^l-17

(Pfeiffer et al., 2002)

wg^UAS.GFP/Scer\GAL4^wg.PM rescues wg^l-17

(Pfeiffer et al., 2002)

Partially rescues

Scer\GAL4^wg-Gal4/wg^UAS.GFP partially rescues wg^Gal4/wg^l-17

(Won et al., 2019)

Comments

The cuticle pattern of wg^l-17 embryos almost completely rescued by the expression of wg^{Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^wg.PM or Scer\GAL4^en-e16E.

(Pfeiffer et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

wg^{Scer\UAS.T:Avic\GFP}

wg^UAS.GFP

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

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