FlyBase Allele Report: Scer\GAL4[c42]

Drives expression in adult Malpighian tubule structures, including principal cells of the main segment, in bar-shaped cells of the initial and transitional segments, and the lower tubule. In the adult brain, drives expression in the ellipsoid and fan-shaped bodies and pars intercerbralis, in about 13 ring neurons R2 and R4m, and in a subset of fan-shaped neurons.

(FlyBase Curators, 2017)

ScerGAL4^c42 labeling is detected in about 13 ring neurons R2 and R4m at 7-8 days of age.

(Martín-Peña et al., 2014)

ScerGAL4^c42 drives expression in Malphighian tubule type I cells (principal cells).

(Terhzaz et al., 2010)

In adult Malpighian tubule, expressed in principal cells of the main segment, in bar-shaped cells of the initial and transitional segments, and in the lower tubule.

(Rosay et al., 1997)

Marker for

Malpighian tubule Type I cell

(Söderberg et al., 2011)

adult ellipsoid body ring neuron

(Renn et al., 1999)

ellipsoid body

(Renn et al., 1999)

fan-shaped body

(Renn et al., 1999)

fan-shaped body tangential neuron

(Renn et al., 1999)

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | larval stage, with Ggt-1^RNAi.UAS

(Liu et al., 2014)

abnormal body size | larval stage, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

abnormal body size | P-stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

abnormal developmental rate, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

abnormal locomotor behavior, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

abnormal oxidative stress response, with sesB^RNAi.UAS

(Terhzaz et al., 2010)

abnormal stress response, with salt^RNAi.WIZ.UAS

(Stergiopoulos et al., 2009)

decreased cell number, with Dronc^pro.UAS

(Shukla and Tapadia, 2011)

decreased cell number | larval stage, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

lethal, with grim^UAS.cUa

(Shukla and Tapadia, 2011)

lethal, with hid^UAS.cUa

(Shukla and Tapadia, 2011)

lethal - all die before end of first instar larval stage, with Dronc^ΔN.UAS

(Shukla and Tapadia, 2011)

lethal - all die before end of first instar larval stage, with rpr^UAS.C

(Shukla and Tapadia, 2011)

lethal - all die before end of larval stage, with pdm3^UAS.CFP

(Chen et al., 2012)

lethal - all die during first instar larval stage, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

lethal - all die during P-stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

partially lethal - majority die, with Dronc^pro.UAS

(Shukla and Tapadia, 2011)

partially lethal - majority die, with Myc^UAS.cZa

(Gamberi et al., 2017)

some die during first instar larval stage, with Dronc^ΔN.UAS

(Shukla and Tapadia, 2011)

some die during first instar larval stage, with rpr^UAS.C

(Shukla and Tapadia, 2011)

some die during larval stage, with pdm3^UAS.CFP

(Chen et al., 2012)

visible, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

Phenotype Manifest In

adult abdomen | P-stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

adult head | P-stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

adult main segment of Malpighian tubule | pharate adult stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

adult Malpighian tubule, with fray^RNAi.UAS

(Wu et al., 2014)

adult Malpighian tubule, with Myc^UAS.cZa

(Gamberi et al., 2017)

adult Malpighian tubule, with Nha1^UAS.EYFP

(Day et al., 2008)

adult Malpighian tubule, with Wnk^{D420A.UAS.cWa}

(Wu et al., 2014)

adult Malpighian tubule, with Wnk^KK102654

(Wu et al., 2014)

adult Malpighian tubule principal cell | pharate adult stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

adult thorax | P-stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

embryonic/first instar larval cuticle, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

embryonic/larval Malpighian tubule, with Dronc^pro.UAS

(Shukla and Tapadia, 2011)

embryonic/larval Malpighian tubule, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

embryonic/larval Malpighian tubule, with sesB^RNAi.UAS

(Terhzaz et al., 2010)

embryonic/larval Malpighian tubule | third instar larval stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

embryonic/larval Malpighian tubule Type I cell, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

embryonic/larval Malpighian tubule Type I cell | third instar larval stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

embryonic/larval Malpighian tubule Type II cell, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

larval adipose system, with EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

Malpighian tubule, with 5-HT7^UAS.cKa

(Kerr et al., 2004)

Malpighian tubule, with Besp\bPAC^UAS.cSa

(Efetova et al., 2013)

Malpighian tubule, with Besp\bPAC^UAS.cSa, Scer\GAL4^tsh-c724

(Efetova et al., 2013)

Malpighian tubule, with Btau\PDE5A^UAS.cBa

(Broderick et al., 2004)

Malpighian tubule, with Oatp58Db^RNAi.UAS

(Torrie et al., 2004)

Malpighian tubule, with Pka-C1^RNAi.UAS.cUa

(Efetova et al., 2013)

Malpighian tubule, with SPoCk^{A.UAS.Tag:MYC}

(Southall et al., 2006)

Malpighian tubule, with SPoCk^RNAi.UAS.WIZ

(Southall et al., 2006)

Malpighian tubule | conditional, with Besp\bPAC^UAS.cSa

(Efetova et al., 2013)

Malpighian tubule | drug conditional, with Rnor\Npr1^UAS.cKa

(Kerr et al., 2004)

Malpighian tubule | P-stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

mitochondrion | larval stage, with sesB^RNAi.UAS

(Terhzaz et al., 2010)

nucleus | pharate adult stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

nucleus | third instar larval stage, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

pupal tracheal section, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

puparium cuticle, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Yadav and Tapadia, 2016)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Besp\bPAC^UAS.cSa, Scer\GAL4^c42 has Malpighian tubule | conditional phenotype, enhanceable by Epac^UAS.cEa, Scer\GAL4^c42

(Efetova et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^c42, Wnk^KK102654 has adult Malpighian tubule phenotype, suppressible by Scer\GAL4^c42/fray^T206E.UAS

(Wu et al., 2014)

Scer\GAL4^c42, Wnk^KK102654 has adult Malpighian tubule phenotype, suppressible by Ncc69^r2

(Wu et al., 2014)

Scer\GAL4^c42, Wnk^KK102654 has adult Malpighian tubule phenotype, suppressible by Scer\GAL4^c42/fray^RNAi.UAS

(Wu et al., 2014)

Enhancer of

Statement

Reference

Epac^UAS.cEa, Scer\GAL4^c42 is an enhancer of Malpighian tubule | conditional phenotype of Besp\bPAC^UAS.cSa, Scer\GAL4^c42

(Efetova et al., 2013)

Suppressor of

Statement

Reference

Myc^JF01761, Scer\GAL4^c42, Scer\GAL4^tsh-c724 is a suppressor of adult Malpighian tubule phenotype of BicC⁵

(Gamberi et al., 2017)

Myc^JF01761, Scer\GAL4^c42, Scer\GAL4^tsh-c724 is a suppressor of adult specialized Malpighian tubule cell phenotype of BicC⁵

(Gamberi et al., 2017)

Myc^JF01762, Scer\GAL4^c42, Scer\GAL4^tsh-c724 is a suppressor of adult Malpighian tubule phenotype of BicC⁵

(Gamberi et al., 2017)

Myc^JF01762, Scer\GAL4^c42, Scer\GAL4^tsh-c724 is a suppressor of adult specialized Malpighian tubule cell phenotype of BicC⁵

(Gamberi et al., 2017)

Scer\GAL4^c42/fray^T206E.UAS is a suppressor of adult Malpighian tubule phenotype of Scer\GAL4^c42, Wnk^KK102654

(Wu et al., 2014)

Scer\GAL4^c42/fray^RNAi.UAS is a suppressor of adult Malpighian tubule phenotype of Scer\GAL4^c42, Wnk^KK102654

(Wu et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^c42/L2HGDH^UAS.cLa rescues L2HGDH¹²/L2HGDH¹⁴

(Mahmoudzadeh et al., 2024)

Scer\GAL4^c42/Ggt-1^UAS.cLa rescues Ggt-1^Δ1523

(Liu et al., 2014)

Scer\GAL4^c42/homer^UAS.Tag:MYC rescues homer^R102

(Urizar et al., 2007)

Partially rescues

Scer\GAL4^c42/EcR^A.UAS partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

EcR^B1.UAS/Scer\GAL4^c42 partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

Scer\GAL4^c42/EcR^B2.UAS partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

Fails to rescue

Scer\GAL4^c42/ERR^UAS.cTa fails to rescue ERR²/ERR¹

(Tennessen et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

30835

w^*; P{GawB}c42

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

GAL4^c42

Scer\GAL4^c42

Name Synonyms

Secondary FlyBase IDs

References (67)

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