FlyBase Allele Report: Dmel\Egfr[top-EC20]

General Information

Symbol

Dmel\Egfr^top-EC20

Species

D. melanogaster

Name

FlyBase ID

FBal0033669

Feature type

allele

Associated gene

Dmel\Egfr

Associated Insertion(s)

Carried in Construct

Also Known As

top^EC20, der^EC20

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Clifford and Schupbach, 1989)

Mutagen

ethyl methanesulfonate

(Clifford and Schupbach, 1994, Clifford and Schupbach, 1989)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G1513A. Amino acid replacement: D382N.

(Clifford and Schupbach, 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:21,555,955..21,555,955 [+]

Nucleotide change:

G21555955A

Reported nucleotide change:

G1513A

Amino acid change:

D382N | Egfr-PA; D431N | Egfr-PB

Reported amino acid change:

D382N

(Clifford and Schupbach, 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity (with Egfr^t1)

(Gaengel and Mlodzik, 2003)

lethal

(Baker and Rubin, 1992)

lethal - all die before end of pupal stage | recessive

(Clifford and Schupbach, 1994, Clifford and Schupbach, 1989)

some die during pupal stage | recessive

(Clifford and Schupbach, 1994, Clifford and Schupbach, 1989)

Phenotype Manifest In

ommatidium (with Egfr^t1)

(Gaengel and Mlodzik, 2003)

Detailed Description

Statement

Reference

Egfr^t1/Egfr^top-EC20 animals display rotation defects in adult ommatidia.

(Gaengel and Mlodzik, 2003)

Enhances the female sterility and adult morphological defects of Egfr^t1. Rarely survives as transheterozygote with the semi-viable Egfr^top-CA allele.

(Clifford and Schupbach, 1994)

Does not suppress the "Ellipse" phenotype. Hemizygotes die during pupariation, and have abnormally small dorsal imaginal discs.

(Baker and Rubin, 1992)

Homozygous embryos show no defects, hemizygotes are pupal lethal. When in combination with Df(2R)Egfr3 the eye-antennal, wing and haltere imaginal discs give rise to eyes, wings and halteres that are reduced size due to altered development of the imaginal disc. Embryos produced from heteroallelic combination with Egfr^t1 have a severe ventralised phenotype, reduction in size of their dorsal appendage.

(Clifford and Schupbach, 1989)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Egfr[+]/Egfr^top-EC20 is a non-enhancer of anterior crossvein phenotype of Scer\GAL4^en-e16E, Socs36E^UASp.cCa

(Callus and Mathey-Prevot, 2002)

Other

Statement

Reference

Egfr^top-EC20, Scer\GAL4^en-e16E, Socs36E^UASp.cCa has wing vein | ectopic phenotype

(Callus and Mathey-Prevot, 2002)

Egfr^top-EC20, Scer\GAL4^en-e16E, Socs36E^UASp.cCa has wing vein L4 phenotype

(Callus and Mathey-Prevot, 2002)

Egfr[+]/Egfr^top-EC20, Scer\GAL4^en-e16E, Socs36E^UASp.cCa has wing vein | ectopic phenotype

(Callus and Mathey-Prevot, 2002)

Egfr[+]/Egfr^top-EC20, Scer\GAL4^en-e16E, Socs36E^UASp.cCa has wing vein L4 phenotype

(Callus and Mathey-Prevot, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Socs36E^{Scer\UAS.P\T.cCa} under the control of Scer\GAL4^en-e16E in a Egfr^top-EC20/+ background results in ectopic wing vein material in 86% of flies and occasional thinning of a section of the L4 longitudinal wing vein. The anterior crossvein loss phenotype of Socs36E^{Scer\UAS.P\T.cCa}-expressing flies is not affected.

(Callus and Mathey-Prevot, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Mutation of Egfr that coordinately affects all gene activities, a class I lesion. The allelic series for class I lesions: Egfr^t2 = Egfr^t1 < Egfr^top-EC20 < Egfr^f7 = Egfr^f1 = Egfr^flb-2E07 < Egfr^top-EE39 = Egfr^top-ED26 = Egfr^f5 < Egfr^f9 = Egfr^f10 = Egfr^f2 = Egfr^top-EE42 = Egfr^f11 = Egfr^f24 = Egfr^f3 = Df(2R)Egfr3.

(Clifford and Schupbach, 1989)

Class I allele.

(Clifford and Schupbach, 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

DER^EC20

(Schnepp et al., 1996)

Egfr^EC20

(Gaengel and Mlodzik, 2003)

Egfr^top-EC20

der^EC20

(Callus and Mathey-Prevot, 2002)

top^EC20

(Clifford and Schupbach, 1994, Baker and Rubin, 1992, Clifford and Schupbach, 1989)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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