FlyBase Allele Report: Dmel\CycA[3]

General Information

Symbol

Dmel\CycA³

Species

D. melanogaster

Name

FlyBase ID

FBal0002201

Feature type

allele

Associated gene

Dmel\CycA

Associated Insertion(s)

Carried in Construct

Also Known As

l(3)183, cycA¹⁸³

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Greaves et al., 1999)

Mutagen

ethyl methanesulfonate

(Lehner and O'Farrell, 1989)

Progenitor genotype

Caused by aberration

Df(3L)CycA³

Cytology

Description

A large deletion that removes the complete CycA locus and extends into flanking genes.

(Lehner, 2013.1.1)

Deletion of CycA coding sequence.

(Lehner et al., 1991)

Small deletion that removes cyclin sequences.

(Lehner and O'Farrell, 1989)

molecular deletion

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Berger et al., 2005, Luer et al., 1997, Lehner et al., 1991)

abnormal mitotic cell cycle | embryonic stage (with CycA⁵)

(Reber et al., 2006)

abnormal neuroanatomy

(Berger et al., 2005)

decreased cell number | embryonic stage

(Akiyama-Oda et al., 2000)

decreased occurrence of cell division | embryonic stage

(Knoblich and Lehner, 1993)

lethal | recessive

(Lehner and O'Farrell, 1989)

Phenotype Manifest In

Detailed Description

Statement

Reference

CycA⁵/CycA³ embryos show a failure of epidermal cells to undergo mitosis 16.

(Reber et al., 2006)

In CycA³ mutant embryos, division of the NB6-4t lineage is arrested afer the first or second division. However, the first division of this lineage produces one glial cell and one neuronal cell as in wild-type.

(Berger et al., 2005)

Only one, two or three cells are found in the position of the NB7-3 clusters (8%, 75% and 17% respectively) compared to the four seen in wild-type.

(Novotny et al., 2002)

In mutant embryos, two to four progeny cells of NB6-4T appear in each hemisegment, as opposed to the 5 to 6 cells seen in wild-type. In the abdominal segment of this mutant, more than half of NB6-4A divided once into 2 cells. The first cell division. the onset of glial differentiation and cell fate bifurcation occur normally in these embryos.

(Akiyama-Oda et al., 2000)

Mitosis is arrested during the 16th cell cycle. Cells are stillable to differentiate and dorsal medial cells carrying bilateral cytoplasmic extensions are clearly identifiable in late embryos. Scer\GAL4^sim.PS-mediated expression of spi^s.Scer\UAS increases the number of dorsal medial cells, the implantation of one or two midline cells from these donors into CycA mutant embryos fails to induce additional dorsal medial cells.

(Luer et al., 1997)

In embryos double mutant for CycA³, no mitotic figures are observed at mitosis 15. BrdU incorporations reveal that the block is in the preceding G₂.

(Knoblich and Lehner, 1993)

CycA^hari/CycA³ heterozygotes exhibit a 'hari' phenotype.

(Ueda et al., 1992)

Entry into mitosis 16 does not occur in the mutant cells of the epidermis. Cells do not exhibit complete cell cycle arrest.

(Lehner et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

CycA³ is an enhancer of visible phenotype of Scer\GAL4^en-e16E, arm^UAS.cWa

(Greaves et al., 1999)

NOT Enhancer of

Statement

Reference

CycA³ is a non-enhancer of visible phenotype of Scer\GAL4^en-e16E, shg^{i.UAS.Tag:SS(aos),Tag:MYC}

(Greaves et al., 1999)

Suppressor of

Statement

Reference

CycA³/CycA[+] is a suppressor of partially lethal phenotype of dally⁰⁶⁴⁶⁴

(Nakato et al., 2002)

CycA³/CycA[+] is a suppressor of partially lethal phenotype of dally^ΔP-305

(Nakato et al., 2002)

CycA³/CycA[+] is a suppressor of visible phenotype of dally⁰⁶⁴⁶⁴

(Nakato et al., 2002)

CycA³ is a suppressor of visible phenotype of rux³

(Thomas et al., 1994)

NOT Suppressor of

Statement

Reference

CycA³/CycA[+] is a non-suppressor of increased cell death phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

CycA³ is a non-suppressor of visible phenotype of Scer\GAL4^en-e16E, shg^{i.UAS.Tag:SS(aos),Tag:MYC}

(Greaves et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

CycA³ has mitotic cell cycle phenotype, suppressible by CycB^hs.PK

(Knoblich and Lehner, 1993)

Enhancer of

Statement

Reference

CycA³ is an enhancer of wing phenotype of Scer\GAL4^en-e16E, arm^UAS.cWa

(Greaves et al., 1999)

NOT Enhancer of

Statement

Reference

CycA³ is a non-enhancer of wing phenotype of Scer\GAL4^en-e16E, shg^{i.UAS.Tag:SS(aos),Tag:MYC}

(Greaves et al., 1999)

Suppressor of

Statement

Reference

CycA³/CycA[+] is a suppressor of ommatidium phenotype of dally⁰⁶⁴⁶⁴

(Nakato et al., 2002)

CycA³/CycA[+] is a suppressor of wing vein L5 phenotype of dally⁰⁶⁴⁶⁴

(Nakato et al., 2002)

CycA³/CycA[+] is a suppressor of antenna phenotype of dally⁰⁶⁴⁶⁴

(Nakato et al., 2002)

CycA³/CycA[+] is a suppressor of lamina anlage & mitotic cell cycle phenotype of dally⁰⁶⁴⁶⁴

(Nakato et al., 2002)

CycA³ is a suppressor of eye phenotype of rux³

(Thomas et al., 1994)

NOT Suppressor of

Statement

Reference

CycA³/CycA[+] is a non-suppressor of eye phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

CycA³/CycA[+] is a non-suppressor of ommatidium phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

CycA³ is a non-suppressor of wing phenotype of Scer\GAL4^en-e16E, shg^{i.UAS.Tag:SS(aos),Tag:MYC}

(Greaves et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The lethality seen in dally⁰⁶⁴⁶⁴ homozygotes is completely suppressed if they are also heterozygous for CycA³. The penetrance of the eye, antenna and wing defects seen in dally⁰⁶⁴⁶⁴ homozygotes is reduced if they are also heterozygous for CycA³. The severity of the dally⁰⁶⁴⁶⁴ eye defects are also reduced in the double mutants. The lethality seen in dally^ΔP-305 homozygotes is reduced if they are also heterozygous for CycA³. The ability of dally⁰⁶⁴⁶⁴ lamina precursor neurons to enter a second division cycle is largely restored if they are also carrying CycA³.

(Nakato et al., 2002)

No effect on the faf eye phenotype.

(Huang and Fischer-Vize, 1996)

Dominantly restores 81% of ommatidia of rux³ to wild type.

(Thomas et al., 1994)

The mitotic cycle phenotype can be rescued by heat shock induced expression of CycB^hs.PK.

(Knoblich and Lehner, 1993)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

CycA³ is rescued by CycA^hs.PK

(Knoblich and Lehner, 1993)

Comments

This phenotype can be rescued by heat shock induced expression of CycA^hs.PK.

(Knoblich and Lehner, 1993)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CycA¹⁸³

(Reber et al., 2006)

CycA³

(Liaw and Chiang, 2019, Matsuda et al., 2015, Lehner, 2013.1.1)

cycA¹⁸³

(Akiyama-Oda et al., 2000, Greaves et al., 1999)

cycA³

(Nakato et al., 2002, Luer et al., 1997)

cycA^l(3)183

(Thomas et al., 1994)

l(3)183

(Lehner, 2013.1.1, Tanaka-Matakatsu et al., 2007, Ohlmeyer and Schupbach, 2003, Huang and Fischer-Vize, 1996, Ueda et al., 1992, Lehner et al., 1991, Lehner and O'Farrell, 1989)

Name Synonyms

Secondary FlyBase IDs

References (20)

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