PRIM2

DNA primase large subunit in humans is encoded by the PRIM2 gene and is part of the enzyme DNA polymerase alpha.

PRIM2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3L9Q, 3Q36, 4BPU, 4BPW, 4BPX, 4RR2, 5F0Q, 5EXR, 5F0S
Identifiers
Aliases	PRIM2, PRIM2A, p58, primase (DNA) subunit 2, DNA primase subunit 2
External IDs	OMIM: 176636; MGI: 97758; HomoloGene: 731; GeneCards: PRIM2; OMA:PRIM2 - orthologs
Gene location (Human) Chr. Chromosome 6 (human) Band 6p11.2 Start 57,314,805 bp End 57,646,850 bp
Gene location (Mouse) Chr. Chromosome 1 (mouse) Band 1 12.7 cM|1 B Start 33,492,891 bp End 33,708,876 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in buccal mucosa cell gonad ganglionic eminence Achilles tendon ventricular zone testicle oocyte epithelium of colon stromal cell of endometrium rectum Top expressed in fetal liver hematopoietic progenitor cell secondary oocyte genital tubercle maxillary prominence primary oocyte mandibular prominence medial ganglionic eminence tail of embryo cumulus cell spermatocyte More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA-directed 5'-3' RNA polymerase activity transferase activity DNA binding 4 iron, 4 sulfur cluster binding iron-sulfur cluster binding nucleotidyltransferase activity metal ion binding single-stranded DNA binding DNA-directed DNA polymerase activity DNA primase activity protein binding Cellular component nucleoplasm alpha DNA polymerase:primase complex Biological process DNA replication DNA replication, synthesis of RNA primer DNA biosynthetic process telomere maintenance via semi-conservative replication G1/S transition of mitotic cell cycle DNA replication initiation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5558 19076 Ensembl ENSG00000146143 ENSMUSG00000026134 UniProt P49643 P33610 RefSeq (mRNA) NM_000947 NM_001282487 NM_001282488 NM_008922 RefSeq (protein) NP_000938 NP_001269416 NP_001269417 NP_032948 Location (UCSC) Chr 6: 57.31 – 57.65 Mb Chr 1: 33.49 – 33.71 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the large, 58 kDa primase subunit.

Further reading

• Stadlbauer F, Brueckner A, Rehfuess C, et al. (1994). "DNA replication in vitro by recombinant DNA-polymerase-alpha-primase". Eur. J. Biochem. 222 (3): 781–93. doi:10.1111/j.1432-1033.1994.tb18925.x. PMID 8026492.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Coll JM, Hickey RJ, Cronkey EA, et al. (1998). "Mapping specific protein-protein interactions within the core component of the breast cell DNA synthesome". Oncol. Res. 9 (11–12): 629–39. PMID 9563011.
• Schneider A, Smith RW, Kautz AR, et al. (1998). "Primase activity of human DNA polymerase alpha-primase. Divalent cations stabilize the enzyme activity of the p48 subunit". J. Biol. Chem. 273 (34): 21608–15. doi:10.1074/jbc.273.34.21608. PMID 9705292.
• Arezi B, Kirk BW, Copeland WC, Kuchta RD (1999). "Interactions of DNA with human DNA primase monitored with photoactivatable cross-linking agents: implications for the role of the p58 subunit". Biochemistry. 38 (39): 12899–907. doi:10.1021/bi9908991. PMID 10504261.
• Smith RW, Nasheuer HP (2002). "Control of complex formation of DNA polymerase alpha-primase and cell-free DNA replication by the C-terminal amino acids of the largest subunit p180". FEBS Lett. 527 (1–3): 143–6. doi:10.1016/S0014-5793(02)03197-6. PMID 12220650. S2CID 27852377.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Mungall AJ, Palmer SA, Sims SK, et al. (2003). "The DNA sequence and analysis of human chromosome 6". Nature. 425 (6960): 805–11. Bibcode:2003Natur.425..805M. doi:10.1038/nature02055. PMID 14574404.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157.

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human DNA primase large subunit (PRIM2)