peritoneum

To test the pathogenicity of strain, lab animal (mice) has been selected and 0.5mL growth suspension having 1x109cfu/mL of each sample was injected intra peritoneally. The post-mortem of the dead animal was performed (Haq et al., 2016).

Balb/c mice (6-8 weeks) were assigned randomly to three experimental groups: (a) sham control group, injected peritoneally with DMSO for 4 weeks once a week; (b) TAC group, injected peritoneally with DMSO for 4 weeks once a week; and (c) TAC group, injected peritoneally with EPZ005687 (10 mg [kg.sup.-1]) for 4 weeks once a week.

40 mice were randomly divided into 4 groups: D-gal group, mice were administrated with D-gal (120 mg/kg-bw), qd x 42 by subcutaneous injection; D-gal + ASP group, from 8th day of D-gal injection, the mice were given ASP (200 mg/kg-bw), qd x 35 at the same time by peritoneal injection; D-gal + VitE group (positive control), from 8th day of D-gal injecting, the mice were injected with Vitamin E (100 mg/kg-bw), qd x 35 at the same time by peritoneal injection; Control group, the mice were injected subcutaneously with saline at the same volume with D-gal for 42 days and peritoneally injected with saline at the same volume with ASP from the 8th day of the treatment.

The induced diabetic mice model was created by injecting alloxan monohydrate (150 mg/kg) intra peritoneally for a single time.

When peritoneally disseminated tumour cells are implanted into these spaces, these may mimic intrahepatic focal lesions.

The knot was placed underneath peritoneally, rather than subcutaneously.

In writhing test, a noxious chemical stimulus (acetic acid) administered intra- peritoneally resulted in abdominal constriction [18].