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Chemical synthesis generally preferred acetic anhydride and vinyl acetate as esterifying agents.
A typical process for preparing CDCA is as follows: esterifying of saponified bile acid, acetylating of bile acid ester, removal of intermediate product by using organic solvent, crystallizing of acetylated ester of CDCA, deprotecting, and crystallizing in organic solvent.
The limitation of this calculation, which is based on the esterifying agent and transesterification catalyst, is that biodiesel production is more economical if pre-treatment of the raw material occurs at the processing site.