fibroblast

Stewart et al., "An autogeneic feeder cell system that efficiently supports growth of undifferentiated human embryonic stem cells," Stem Cells, vol.

A wide range of feeder cell types, conditioned media, and feeder-free systems has been investigated [25, 34], including a xeno-free system for derivation of hESCs using human serum and a human foreskin fibroblast feeder layer [69].

Interestingly, reprogramming by MEFs tended to retain trimethyl-histone H3 (Lys27) compared with iPSCs established on SL10, again suggesting that the feeder cell is one factor that affects the XCI state (Supplementary Figure 6).

The antigen is present in five hES cell lines, but does not appear to be expressed on differentiated or feeder cell types.

The first of these efforts (30) resulted in an immortal epithelial cell culture that was easy to grow on serum-containing growth medium, required no substrate other than plastic for attachment, required no irradiated feeder cell layer, and had a generation time similar to that of primary cultures of human urothelial cell cultures.

Thus, these data demonstrated that the DF-1 feeder was the optimal feeder cell for the culture of cESCs in vitro.

We observed blastocyst surface morphology and blastocysts attaching onto the feeder cell layer under high magnification to identify the early outgrowth process in porcine blastocysts.

HepaLife, Inc., Vancouver, British Columbia, announced that its patented liver cells demonstrate ability to function and survive without feeder cell support, following successful room temperature and human plasma survival outcomes.

Hoechst staining of H9 cells was less intense than in the Fed1A feeder cell layer, indicative of a "Hoechst dim" phenotype due to efflux of Hoechst dye by stem cells, in contrast to brighter staining observed on the feeder layer (Figures 2(a-ii) and 2(b-ii)).

After the 3 rounds of subculture, a sufficient number of d-hES cells were obtained for feeder cell preparation.