Tissue for ultrastructural analysis was fixed promptly after excision in 2.5% glutaraldehyde buffered with 0.1 mol/L cacodylate buffer, pH 4.3, postfixed in buffered 1% osmium
tetroxide, block stained with 2% uranyl acetate, and embedded in spur resin.
Sperm on the paper were fixed in 2% glutaraldehyde in 70% seawater, followed by postfixation with 2% osmium
tetroxide in 2.5% NaHC[O.sub.3].
Lung parenchyma with multiple fat emboli (hematoxylineosin with osmium
tetroxide overstaining, original magnification X10).
The samples were cut into 4 [mm.sup.*] 4 [mm.sup.*] 2 mm and fixed with 2.5% glutaraldehyde, rinsed in the 0.1 M phosphate buffer (pH 7.4), and postfixed in 1% of osmium
tetroxide at 4[degrees]C for 2h before being dehydrated with graded ethanol and acetone.
Hydroxyl and hydrogen
tetroxide (H[O.sub.4]) as chain intermediates," Journal of Physical Chemistry, vol.
After postfixing in 2% osmium
tetroxide at 4[degrees]C for 1 h, cochleae were dehydrated and embedded in 812 resin.
Samples were also post-fixed in 1 % sodium cacodyl buffered osmium
tetroxide, pH 7.2 for 1 h.
Tissues were prepared for Scanning Electron Microscopy (SEM) by washing in distilled water, post-fixation in osmium
tetroxide 1% (OsO4) for 2 hours, and dehydration using ethanol solutions with increasing concentrations (from 50%) until reaching the critical point (QUORUM/K850).
On Hypovanadic Oxide (Vanadium
Tetroxide), and its compounds / J.K.
For scanning electron microscopy (SEM), 2 parasites were washed five times with 0.2 M cacodylate buffer (pH 7.3), fixed in 2.5% glutaraldehyde and post fixed in 1% osmium
tetroxide at 4degC.
Worms processed for SEM were cleaned in saline preserved in 1.25% gluteraldehyde, post-fixed in 1% osmium
tetroxide, dehydrated in an ethanol series, critical point dried using C[O.sub.2], mounted on stubs, coated with gold palladium alloy and viewed with a Jeol (Model JSM 35-C) scanning electron microscope.
Small scaffold supports were fixed in 4% (v/v) paraformaldehyde and postfixed in 2% osmium
tetroxide. After washing with 0.1 M phosphate buffer, the sample was dehydrated by a series of incubations in 30%, 50%, and 70% (v/v) ethanol.
Another portion of these fragments was fixed in paraformaldehyde 4% and postfixed in osmium
tetroxide, processed for histological technique routine, and subjected to cross sections of 5 [micro]m, for performing the morphometric analysis [16].
