To determine if type 1 pilus binding to mannose receptors affected fim gene expression, a LD-RT-PCR assay was performed.
Type 1 Pilus Expression Changes after Ligand-Receptor Binding.
The company also agreed
Pilus would pay to Tauriga 20% of its net profit to Tauriga.
Pilus protein-specific IgG was measured by multiplex immunoassay that used recombinant pilus proteins BP-1, BP-2a, and BP-2b coupled to magnetic beads and expressed as relative Luminex units (RLU) per milliliter.
Standard serum for determination of human IgG specific for GBS pilus proteins was obtained by pooling hyperimmune serum from persons colonized by GBS.
As a result of the transaction, the buyer also acquired a portfolio of global intellectual property (IP) assets from Bacterial Robotics LLC,
Pilus' parent, and a microbial fuel cell patent issued to the target in January 2013.
It has been shown that one ST25 isolate from Canada, which does not have a discrete srtF
pilus cluster and is unable to express the srtF
pilus, is nonetheless PCR positive for each of the individual srtF genes because PCR amplicons can be generated from homologs of these genes found at various genome locations (13,16).
24 December 2013 - US diversified company Tauriga Sciences Inc (OTCMKTS:TAUG) said today that it was committed to completing the planned purchase of local alternative cleantech energy solutions developer
Pilus Energy LLC, despite the recent downward trend in target's stock price.
The PI-2
pilus is composed of polymers of the major
pilus protein PitB (9).
In addition, the authors describe in great detail the recognition of host receptors by pili and the
pilus biogenesis by chaperon-user pathways.
Finally, both strains carried the bfpA gene encoding bundlin, the structural subunit of the bundle-forming
pilus expressed by typical EPEC strains but not by EHEC.
An inducible bundle-forming
pilus produced by enteropathogenic Escherichia coli.
Pilus and non-pilus bacterial adhesins: assembly and function in cell recognition.