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    You say you only want the entries that are not found in either column1 or 2 from file2, but your "desired output" includes the entries ENSG00000028203 and ENSG00000177051, which are found in the first column, on the 4th and 2nd rows respectively. Commented Aug 28, 2019 at 16:18
  • Sorry, it was manual editing and i missed that ..I corrected the desired output. Commented Aug 28, 2019 at 17:35