2014
Activity of Tea Tree (Melaleuca alternifolia) Essential Oil against L3 Larvae ofAnisakis simplex
Abstract: Nematicidal activity of Melaleuca alternifolia essential oil, commonly known as tea tree oil (TTO), was assayed in vitro against L3 larvae of Anisakis simplex. The results showed a mortality of 100% for concentrations between 7 and 10 μL/mL after 48 h of incubation, obtaining an LD50 value of 4.53 μL/mL after 24 hours and 4.27 μL/mL after 48 hours. Concentration-dependent inhibition of acetylcholinesterase was observed for tea tree essential oil showing inhibition values of 100% at 100 μL/mL. This fact suggest…
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Cited by 33 publications
(27 citation statements)
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“…This study confirms the anisakicidal activity reported for natural plants and spices used for our olive oil aromatization and also for their major compounds (β‐pinene, γ‐terpinene, 1,8‐cineole, p ‐cymene, eugenol, and limonene) . The different types of lesions observed in all dead parasites are related to the different active components, in agreement with the literature .…”
Section: Resultssupporting
confidence: 91%
“…This study confirms the anisakicidal activity reported for natural plants and spices used for our olive oil aromatization and also for their major compounds (β‐pinene, γ‐terpinene, 1,8‐cineole, p ‐cymene, eugenol, and limonene) . The different types of lesions observed in all dead parasites are related to the different active components, in agreement with the literature .…”
Section: Resultssupporting
confidence: 91%
“…In our study, the essential oil from O. syriacum was reported as an effective antiparasitic agent in the fight against anisakiasis, as well as a good larvicidal and adulticidal product to manage mosquito populations. The larvicidal activity of certain essential oils on A. simplex has been established [26,27,28,29,30,31,62]. Though other Origanum species have shown antiparasitic activity on A. simplex , O. syriacum seems to be the most promising, as it induces a higher mortality rate of the larvae at lower concentration.…”
Section: Discussionmentioning
confidence: 99%
“…Ten larvae were introduced in each well of polystyrene six-well plates with a final volume of 2 mL containing different concentrations of the test solution as well as control wells without treatments [27]. O. syriacum essential oil was tested on A. simplex in the range of 0–1 mg mL −1 .…”
Section: Methodsmentioning
confidence: 99%
“…This assay was performed in 6 well plates. Ten larvae were introduced in each well of polystyrene plates with a final volume of 2 ml sterile saline solution containing different concentrations of the test solution (Gómez-Rincón et al 2014 ). O. compactum essential oil was tested against Anisakis in the range of 0–1 μl/ml.…”
Section: Methodsmentioning
confidence: 99%
