Alternative titles; symbols
HGNC Approved Gene Symbol: TAAR5
Cytogenetic location: 6q23.2 Genomic coordinates (GRCh38) : 6:132,588,592-132,616,747 (from NCBI)
Using degenerate oligonucleotides containing the conserved sequence of G protein-coupled receptors, Zeng et al. (1998) obtained a partial genomic DNA. By screening a human lambda DASH II genomic library with this clone, they obtained the full-length gene, which they designated PNR. PNR encodes a deduced 338-amino acid protein with a molecular mass of 38.3 kD. It contains the characteristic 7-transmembrane domain of G protein-coupled receptors and a potential N-glycosylation site. It shares 35% sequence identity with the rat 5-HT-1D receptor (182133) and 34% and 33% sequence identity with the alpha-2-adrenergic receptor (see 104210) and the dopamine D2 receptor (126450), respectively. Northern blot analysis demonstrated almost exclusive expression of a major 4.2-kb PNR transcript in skeletal muscle and selected brain tissues, including amygdala, hippocampus, caudate nucleus, thalamus, and hypothalamus. Weak expression was found in substantia nigra, but no expression was found in corpus callosum or subthalamic nucleus. Some minor transcripts were also found in several brain tissues.
Lindemann et al. (2005) determined that the coding region of TAAR5 is contained within a single exon.
By FISH, Zeng et al. (1998) mapped the PNR gene to chromosome 6q23.
Duan et al. (2004) showed that the PNR gene is located within a gene cluster spanning 132.8 cM on chromosome 6q23.2 which contains all known TRAR genes (e.g., 608282), 3 TRAR pseudogenes, MOXD1, and STX7 (603217).
Duan, J., Martinez, M., Sanders, A. R., Hou, C., Saitou, N., Kitano, T., Mowry, B. J., Crowe, R. R., Silverman, J. M., Levinson, D. F., Gejman, P. V. Polymorphisms in the trace amine receptor 4 (TRAR4) gene on chromosome 6q23.2 are associated with susceptibility to schizophrenia. Am. J. Hum. Genet. 75: 624-638, 2004. [PubMed: 15329799] [Full Text: https://doi.org/10.1086/424887]
Lindemann, L., Ebeling, M., Kratochwil, N. A., Bunzow, J. R., Grandy, D. K., Hoener, M. C. Trace amine-associated receptors form structurally and functionally distinct subfamilies of novel G protein-coupled receptors. Genomics 85: 372-385, 2005. [PubMed: 15718104] [Full Text: https://doi.org/10.1016/j.ygeno.2004.11.010]
Zeng, Z., Fan, P., Rand, E., Kyaw, H., Su, K., Madike, V., Carter, K. C., Li, Y. Cloning of a putative human neurotransmitter receptor expressed in skeletal muscle and brain. Biochem. Biophys. Res. Commun. 242: 575-578, 1998. [PubMed: 9464258] [Full Text: https://doi.org/10.1006/bbrc.1997.7591]