Alternative titles; symbols
HGNC Approved Gene Symbol: ARHGAP5
Cytogenetic location: 14q12 Genomic coordinates (GRCh38) : 14:32,077,304-32,159,728 (from NCBI)
Rho GTPase-activating proteins (Rho-GAPs) negatively regulate the Rho GTPases, a family which appears to mediate many cytoskeleton changes, by stimulating the hydrolysis of bound GTP. Burbelo et al. (1995) isolated human cDNAs with homology to the gene encoding rat p190 Rho-GAP. The predicted 1,499-amino acid human protein, designated p190B by the authors, has an N-terminal GTPase domain and a C-terminal Rho-GAP domain. The amino acid sequences of p190B and rat p190 are 51% identical. In vitro, the Rho-GAP domain of p190B showed GAP activity for several Rho GTPases, including RhoA (165390), RAC1 (602048), and CDC42 (116952). Immunoprecipitation and immunofluorescence studies showed that p190B was localized diffusely in the cytoplasm and in fibrillar patterns that colocalized with the ITGA5 (135620)-ITGB1 (135630) integrin receptor for fibronectin. By Northern blot analysis, Burbelo et al. (1995) detected 4.4- and 6.4-kb p190B transcripts in a variety of tissues.
Stumpf (2024) mapped the ARHGAP5 gene to chromosome 14q12 based on an alignment of the ARHGAP5 sequence (GenBank AK292966) with the genomic sequence (GRCh38).
Burbelo, P. D., Miyamoto, S., Utani, A., Brill, S., Yamada, K. M., Hall, A., Yamada, Y. p190-B, a new member of the Rho GAP family, and Rho are induced to cluster after integrin cross-linking. J. Biol. Chem. 270: 30919-30926, 1995. [PubMed: 8537347] [Full Text: https://doi.org/10.1074/jbc.270.52.30919]
Stumpf, A. M. Personal Communication. Baltimore, Md. 07/16/2024.