Alternative titles; symbols
HGNC Approved Gene Symbol: P2RY4
Cytogenetic location: Xq13.1 Genomic coordinates (GRCh38) : X:70,258,166-70,260,204 (from NCBI)
Nguyen et al. (1995) detected genes encoding novel G protein-coupled receptors (GPCRs) by PCR amplification of human genomic DNA using degenerate oligonucleotides based on the sequences encoding transmembrane spanning domains of the opioid receptors, matostatin receptors, and related GPCRs. They identified a full-length genomic clone containing an intronless open reading frame of 1095 bp encoding a protein of 365 amino acids, features characteristic of GPCRs. The encoded protein, UNR, has greatest overall identity (54%) with human P2U purinoceptor (P2RY2; 600041). The greatest degree of amino acid identity occurs within the putative 7-transmembrane domain, where it demonstrates 74% identity. Dendrogram analysis of the G protein-coupled P2 purinoceptors and UNR indicated that these receptors belong to a family that may be more aptly named nucleotide receptors.
Communi et al. (1995) likewise cloned UNR and recognized it as a novel member of the G protein-coupled P2 purinergic receptor family that functionally behaves as a pyrimidinergic receptor.
Nguyen et al. (1995) found that, when expressed in a mammalian cell line, UNR was activated specifically by UTP and UDP, but not by ATP and ADP. Activation of UNR resulted in increased inositol phosphate formation and calcium mobilization.
Adrian et al. (2000) analyzed the expression of several purinergic receptors during differentiation in a promyelocytic leukemia cell line. Granulocytic differentiation was induced by dimethylsulfoxide, and a monocytic/macrophage phenotype was induced by phorbol esters. P2Y4 was highly expressed in uninduced promyelocytes, and expression decreased slightly following both granulocytic and monocytic differentiation.
By fluorescence in situ hybridization, Nguyen et al. (1995) demonstrated that the UNR gene is located on chromosome Xq13.
Adrian, K., Bernhard, M. K., Breitinger, H.-G., Ogilvie, A. Expression of purinergic receptors (ionotropic P2X1-7 and metabotropic P2Y1-11) during myeloid differentiation of HL60 cells. Biochim. Biophys. Acta 1492: 127-138, 2000. [PubMed: 11004484] [Full Text: https://doi.org/10.1016/s0167-4781(00)00094-4]
Communi, D., Pirotton, S., Parmentier, M., Boeynaems, J.-M. Cloning and functional expression of a human uridine nucleotide receptor. J. Biol. Chem. 270: 30849-30852, 1995. [PubMed: 8537336] [Full Text: https://doi.org/10.1074/jbc.270.52.30849]
Nguyen, T., Erb, L., Weisman, G. A., Marchese, A., Heng, H. H. Q., Garrad, R. C., George, S. R., Turner, J. T., O'Dowd, B. F. Cloning, expression, and chromosomal localization of the human uridine nucleotide receptor gene. J. Biol. Chem. 270: 30845-30848, 1995. [PubMed: 8537335] [Full Text: https://doi.org/10.1074/jbc.270.52.30845]