This Review presents the formation of large extracellular vesicle (L-EV) subsets, highlighting that their contents and physiological relevance, for example, in intercellular communication, are driven by the different mechanisms underlying their biogenesis.

This Perspective highlights the failures of an ageing cell in properly maintaining mRNA health at the various steps of the mRNA life cycle that result in vulnerability to disease, pointing to RNA imbalance as an emerging hallmark of cellular ageing.

This Perspective discusses the mechanisms underlying both the formation and scission of caveolae, with a focus on highlighting the role of specific lipids in regulating and coupling these processes.

This Review discusses the role of organelles, including the ER, mitochondria and lysosomes, as metabolic signalling hubs in ageing and longevity regulation, and highlights their crosstalk that maintains cellular homeostasis and organism fitness.

Mizrak et al. use single-molecule tracking to dissect mechanisms of protein targeting to lipid droplets. They show that nanodomain-based confinement drives selective protein accumulation on lipid droplets.

Wei et al. show that Meg3⁺ haematopoietic stem cells (HSCs) expand upon ageing and present with an increase in H3K23ac levels observed already at middle age. Inhibiting H3K23ac ameliorates megakaryocyte/platelet and myeloid skewing in aged HSCs.

Single-cell transplantations of long-term haematopoietic stem cells (HSCs), in-depth phenotyping and mathematical modelling identify reconstitution kinetics as a unifying metric of HSC potency and extrinsic regulation as a driver of HSC lineage bias.

Ge, Wang, Zhao, Guo et al. report a role for stress granules in suppressing ferroptosis by sequestering ferritin upon irradiation and temozolomide treatment in glioblastoma stem cells, which may potentially be targeted for improved efficacy.

Downes et al. use in situ cryo-electron tomography and super-resolution fluorescence microscopy to define ER exit site architecture in unperturbed human cells. They visualize ribosome-exclusion zones enriched with COPII- and COPI-coated vesicles.

Autorino et al. report a feedback loop between Nodal signalling and tissue phase transitions in zebrafish embryos. Nodal alters adhesion, triggering tissue rigidification and reduced porosity, which limits its diffusion and speeds up Lefty expression.