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Insertional Mutagenesis as a Tool to Study Genes/Functions in Chlamydomonas

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Transgenic Microalgae as Green Cell Factories

Part of the book series: Advances in Experimental Medicine and Biology ((volume 616))

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Abstract

The unicellular alga Chlamydomonas reinhardtii has emerged during the last decades as a model system to understand gene functions, many of them shared by bacteria, fungi, plants, animals and humans. A powerful resource for the research community is the availability of complete collections of stable mutants for studying whole genome function. In the meantime other strategies might be developed; insertional mutagenesis has become currently the best strategy to disrupt and tag nuclear genes in Chlamydomonas allowing forward and reverse genetic approaches. Here, we outline the mutagenesis technique stressing the idea of generating databases for ordered mutant libraries, and also of improving efficient methods for reverse genetics to identify mutants defective in a particular gene.

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Authors and Affiliations

  1. Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Córdoba, Campus de Rabanales, Edificio Severo Ochoa, 14071, Córdoba, Spain

    Aurora Galván

  2. Departamento de Bioquímica y Biología Molecular Campus de Rabanales, Universidad de Códoba, Códoba, Spain

    Aurora Galván, David González-Ballester & Emilio Fernández

  3. Department of Plant Biology The Carnegie Institution of Washington, University of Stanford, Stanford, California, USA

    David González-Ballester

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  1. Aurora Galván
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  2. David González-Ballester
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  1. Departamento de Química y Ciencia de Materiales, Facultad de Ciencias Experimentales, Universidad de Huelva, Huelva, Spain

    Rosa León Ph.D.

  2. Departamento de Bioquímica y Biología Molecular, Campus de Rabanales, Universidad de Córdoba, Córdoba, Spain

    Aurora Galván Ph.D. & Emilio Fernández Ph.D. & 

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Galván, A., González-Ballester, D., Fernández, E. (2007). Insertional Mutagenesis as a Tool to Study Genes/Functions in Chlamydomonas . In: León, R., Galván, A., Fernández, E. (eds) Transgenic Microalgae as Green Cell Factories. Advances in Experimental Medicine and Biology, vol 616. Springer, New York, NY. https://doi.org/10.1007/978-0-387-75532-8_7

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