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| Term | pull down | ID (Ontology) | MI:0096 (Molecular Interactions) | |||||
| Definition | A specific affinity chromatography method where a molecule of interest (bait) is bound to a column, often via an affinity tag expressed as a protein fusion (GST, HIS tag and others) or chemically linked to the bait molecule . The molecule may be expressed or synthesised and purified first, often in an heterologous system, bound to the matrix at high concentration and then challenged with a solution or cellular extract containing the candidate partner molecules. Alternatively, a multi-molecular complex may be adsorbed to the resin and the retained binding molecules subsequently identified.[ PubMed:14755292 ] | |||||||
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chromatography technology__ affinity technology________| affinity chromatography technology |__pull down 3062 rec. |__interactome parallel affinity capture |
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| Is a | affinity chromatography technology | ||
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