FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
EcR, 14-3-3ε
FlyBase ID
FBig0000109393
Interaction Network
Interactions Browser links
EcR network
14-3-3ε network
Reported Interactions
FBrf0235460-1.FRET.FM
Reference
Ng et al., 2017
Description
physical association
Assay
Collection
Source/Stage
fat body / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
EcR 

EcR

fluorescence donor

Alexa 488 tagged secondary Ab

EcR 

EcR

fluorescence donor

Alexa 488 tagged secondary Ab

EcR 

EcR

fluorescence donor

Alexa 488 tagged secondary Ab

14-3-3ε 

14-3-3ε

fluorescence acceptor

Alexa 555 tagged secondary Ab

14-3-3ε 

14-3-3ε

fluorescence acceptor

Alexa 555 tagged secondary Ab

14-3-3ε 

14-3-3ε

fluorescence acceptor

Alexa 555 tagged secondary Ab

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was fat body of wild-type fly line; fluorescence donor produced from endogenous gene; fluorescence acceptor produced from endogenous gene.

(Ng et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)