UASt regulatory sequences drive expression of hid lacking the mitochondria localisation domain (the C-terminal 20 amino acids are deleted). The protein is tagged at the C-terminal end with Tag:FLAG.
Expression of hidΔC20.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4αTub84B.PL leads to lethality; expression under Scer\GAL4ppk.PG does not affect dendritic arborization pattern in class IV dendritic arborizing neurons in third instar larvae.