Constitutive expression of Acf1^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^αTub84B.PL is lethal.

Overexpression of Acf1^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^ey.PH results in gross abnormalities in the adult eye, ranging from areas that lack ommatidia, the outgrowth of antenna-like structures from the eye field, oversized or deformed eyes and antannal duplication. These phenotypes are highly penetrant regardless of the transgene insertion site, as they are observed 65-77% of the adult flies analysed.

About half of the eye imaginal discs show obvious morphological abnormalities and irregularities in photoreceptor cell arrangement upon expression of Acf1^{Scer\UAS.T:Zzzz\FLAG} with the Scer\GAL4^ey.PH driver.

Overexpression of Acf1^{Scer\UAS.T:Zzzz\FLAG} using Scer\GAL4^en.PU or Scer\GAL4^da.G32 drivers results in normal adult fly structures.

No abnormalities result from the overexpression of Acf1^{Scer\UAS.T:Zzzz\FLAG} in post-mitotic photoreceptor cells posterior to the morphogenetic furrow using the Scer\GAL4^GMR.PF driver.

Scer\GAL4^ey.PH>Acf1^{Scer\UAS.T:Zzzz\FLAG}-expression leads to a delay asynchrony of S phase onset along the morphogenetic furrow.

The distribution of apoptotic cells in eye discs expressing Scer\GAL4^ey.PH>Acf1^{Scer\UAS.T:Zzzz\FLAG} is not profoundly different from that of controls. However, in some Scer\GAL4^ey.PH>Acf1^{Scer\UAS.T:Zzzz\FLAG} discs, but never in controls, apoptotic cells are noticed which are aligned parallel to the morphogenetic furrow.

Expression of Acf1^{Scer\UAS.T:Zzzz\FLAG} in salivary gland cells under the control of Scer\GAL4²³¹⁴ probably interferes with heterochromatin formation, because the banding pattern and size of nuclei with polytene chromosomes appear abnormal. Although a certain degree of variability is observed, the nuclei where Acf1^{Scer\UAS.T:Zzzz\FLAG} is expressed appear to be generally larger than in controls.