FlyBase Allele Report: Dmel\CycE[WX]

General Information

Symbol

Dmel\CycE^WX

Species

D. melanogaster

Name

FlyBase ID

FBal0241968

Feature type

allele

Associated gene

Dmel\CycE

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Wang and Kalderon, 2009)

Mutagen

ethyl methanesulfonate

(Wang and Kalderon, 2009)

Progenitor genotype

Cytology

Description

An "AG" to "AA" point mutation at the acceptor site of the last intron-exon junction which results in a frame-shift by one nucleotide. As a result, the last 91 amino acids of the wild-type gene product are changed to 31 new amino acids. The conserved "LTPP" destruction box is missing in CycE^WX the protein.

(Wang and Kalderon, 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:15,729,157..15,729,157 [-]

Nucleotide change:

G15729157A

Comment:

AG to AA mutation in the splice acceptor of the last CycE intron.

(Wang and Kalderon, 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

follicle stem cell | somatic clone

(Wang and Kalderon, 2009)

Detailed Description

Statement

Reference

The percentage of ovarioles that retain CycE^WX mutant follicle stem cell clones is lower than that of wild-type clones. In contrast, there is no significant difference in CycE^WX mutant germ line stem cell retention compared to wild-type clones.

(Wang and Kalderon, 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

CycE^WX has follicle stem cell | somatic clone phenotype, enhanceable by dap^UAS.cdNa/Scer\GAL4^Tub.PU

(Wang and Kalderon, 2009)

Suppressed by

Statement

Reference

CycE^WX has follicle stem cell | somatic clone phenotype, suppressible by dap^unspecified

(Wang and Kalderon, 2009)

CycE^WX has follicle stem cell | somatic clone phenotype, suppressible by E2f1^UAS.cNa/Dp^UAS.cDa/Scer\GAL4^Tub.PU

(Wang and Kalderon, 2009)

CycE^WX has follicle stem cell | somatic clone phenotype, suppressible by shg^UAS.cSa/Scer\GAL4^unspecified

(Wang and Kalderon, 2009)

NOT suppressed by

Statement

Reference

CycE^WX has follicle stem cell | somatic clone phenotype, non-suppressible by Scer\GAL4^Act5C.PI/Scer\GAL4^Tub.PU/Diap1^UAS.cHa

(Wang and Kalderon, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for dap^unspecified (a null allele of dap) partially rescues the persistence of CycE^WX mutant follicle stem cells, measured 12 days after clone induction in both adults and larvae.

Expression of Scer\GAL4^tub-driven dap^{Scer\UAS.cdNa} completely suppresses the formation of large transient CycE^WX follicle cell clones.

Scer\GAL4^tub-driven co-expression of E2f^Scer\UAS.cNa and Dp^Scer\UAS.cDa in follicle stem cell (FSC) clones suppresses the CycE^WX-associated defective FSC-maintenance phenotype.

Scer\GAL4^tub- or Scer\GAL4^Act5C.PI-driven expression of th^Scer\UAS.cHa does not suppress the loss of follicle stem cells in CycE^WX mutant clones.

Scer\GAL4^unspecified-driven expression of shg^Scer\UAS.cSa suppresses the loss of follicle stem cells in somatic clones of CycE^WX.

(Wang and Kalderon, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

CycE^WX is rescued by CycE^UAS.cWa/Scer\GAL4^Tub.PU

(Wang and Kalderon, 2009)

CycE^WX is rescued by CycE^UAS.cWa/Scer\GAL4^Act5C.PI/Scer\GAL4^Tub.PU

(Wang and Kalderon, 2009)