FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\EcRR344Q
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General Information
Symbol
Dmel\EcRR344Q
Species
D. melanogaster
Name
FlyBase ID
FBal0083486
Feature type
allele
Associated gene
Dmel\EcR
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Bender et al., 1997)
Progenitor genotype
Cytology
Description

Amino acid replacement: R?Q.

(Bender et al., 1997)

Mutation is in amino acid 344 of the EcR-B1 open reading frame, just downstream of the DNA-binding domain within a conserved arginine/lysine-rich motif.

(Bender et al., 1997)

Nucleotide substitution: G?A.

(Bender et al., 1997)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:6,094,720..6,094,720 [-]
Nucleotide change:

G6094720A

Reported nucleotide change:

G?A

Amino acid change:

R315Q | EcR-PA; R344Q | EcR-PB; R135Q | EcR-PC; R315Q | EcR-PD; R315Q | EcR-PE; R344Q | EcR-PG

Reported amino acid change:

R344Q

(Bender et al., 1997)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      EcRA483T/EcRR344Q animals survive at 22oC, but not at 25oC. EcRA483T/EcRR344Q females raised at 22o and then shifted to 29oC and mated to wild-type males for 2 days show a nearly 100% decrease in fecundity compared to control siblings. EcRA483T/EcRR344Q females raised at 22o and then shifted to 29oC show an excess of mature stage 14 egg chambers and a decrease in the number of vitellogenic egg chambers between stages 7 and 14 relative to control siblings. Ovarioles containing more than one stage 14 egg chambers in the most posterior portions of the ovariole are seen. Defective egg chambers are also seen. Some defective egg chambers contain very few follicle cells, while others have defects that are limited to nurse cell nuclei. Two types of nurse cell defect are seen; an apparent breakdown of the nuclei of stage 8 and 9 egg chambers and nurse cell nuclei that are dramatically smaller than normal (this defect is seen in both previtellogenic and early vitellogenic egg chambers). Ovaries from EcRA483T/EcRR344Q females have severely reduced numbers of mid- (stage 10) and late (stages 11-13) vitellogenic egg chambers compared to controls. EcRA483T/EcRR344Q females also show a strong reduction in the number of early (stage 8 and 9) vitellogenic egg chambers.

      (Carney and Bender, 2000)

      EcRR344Q/EcRM554fs embryos generally do not hatch.

      (Bender et al., 1997)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      EcRR344Q
      Name Synonyms
      Secondary FlyBase IDs
        References (2)