HSC

The PCR conditions for FUT1 were 95[degrees]C for 2min followed by 30 cycles of 98[degrees]C for 20 s, 60[degrees]C for 5 s, and 72[degrees]C for 30 s; for GAPDH we used 20 cycles.

Determination of the FUT1 Transcription Start Site in DLD-1 Cells Using 5'-Rapid Amplification of cDNA Ends (5'-RACE).

The 5'-flanking region -700 to +1nt of the FUT1 transcriptional start site was amplified from DLD-1-derived genomic DNA by PCR using the forward and reverse primers 5'-AGGTGAGTAGACTCAGGTGGCCTG-3' and 5'-CCCAGGTTCTTTCAGGAGCAC-3', respectively.

Resultados de investigacion muestran que los genes receptor estrogenico (ESR), receptor de la prolactina (PRLR), alpha 1,2 fucosyltransferasa (FUT1) y retinol-binding protein 4 (RBP4) estan asociados con tamano de la camada en algunas razas comerciales [4, 11, 17, 20].

El gen FUT1 es estudiado como gen candidato para resistencia a infecciones causadas por E.

El polimorfismo de los genes ESR, PRLR, FUT1 y RBP4 se determino empleando las tecnicas de PCR-RFLP siguiendo protocolos previamente establecidos por otros investigadores [4, 12, 16, 19].

To determine the FUT1 M307 genotype, we used PCR amplification followed by digestion with Hin6I (MBI).

Chi-square test of the SAS software package (SAS8.0) was applied to determine the association between FUT1 genotype and adhesion phenotype.

A 421 bp fragment was amplified from the FUT1 gene.

The para-Bombay blood group is caused by a deficient FUT1 (191), whereas the non-secretor blood group is caused by a deficient FUT2 (192).

Diagnosis: The diagnosis of FUT1 or FUT2 deficiency can be confirmed by mutation analysis of the genes (191-193).

Missense mutation of FUT1 and deletion of FUT2 are responsible for Indian Bombay phenotype of ABO blood group system.