Skip to main content

Part of the book series: Springer Lab Manual ((SLM))

Abstract

Media consisting of a few chemicals maybe prepared by dissolving these chemicals in solution one after the other. Following is the preparation of MM medium (Mitsuhashi and Maramorosch, 1964) as an example of such media. (For the composition of the medium, see Appendix 1.)

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Chapter
USD 29.95
Price excludes VAT (USA)
eBook
USD 109.00
Price excludes VAT (USA)
Softcover Book
USD 139.99
Price excludes VAT (USA)

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Grace, T.D.C. (1962) Establishment of four strains of cells from insect tissues grown in vitro. Nature 195, 788–789.

    Article  PubMed  CAS  Google Scholar 

  • Maiorella, B., Inlow, D., Shauger, A. and Harano, D. (1988) Large-scale insect cell culture for recombinant protein production. Bio / Technology 6, 1406–1410.

    CAS  Google Scholar 

  • Mitsuhashi, J. (1989) Nutritional requirements of insect cells in vitro. In: Mitsuhashi, J. (ed) Invertebrate Cell System Applications, Vol. I. CRC, Boca Raton, pp 3–20.

    Google Scholar 

  • Mitsuhashi, J. and Maramorosch, K. (1964) Leafhopper tissue culture: Embryonic, nymphal and imaginal tissues from aseptic insects. Contrib. Boyce Thompson Inst. 22, 435–460.

    Google Scholar 

  • Weiss, S.A., Smith, G.C., Kalter, S.S. and Vaughn, J.L. (1981) Improved method for the production of insect cell cultures in large volume. In Vitro 17, 495–502.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

Copyright information

© 2002 Springer Japan

About this chapter

Cite this chapter

Mitsuhashi, J. (2002). Preparation of Media. In: Invertebrate Tissue Culture Methods. Springer Lab Manual. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67875-5_3

Download citation

  • DOI: https://doi.org/10.1007/978-4-431-67875-5_3

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-70313-6

  • Online ISBN: 978-4-431-67875-5

  • eBook Packages: Springer Book Archive

Keywords

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Publish with us

Policies and ethics