Similarly, significant reduction (up to 59%) in
ascorbate peroxidase (cAPX) were also noted by the rhizobacterial inoculation both the presence and absence of L-TRP (Fig.
Ascorbate peroxidase and hydrogen peroxide scavenging enzyme in plants.
Molecular characterization and physiological role of a glyoxysome-bound
ascorbate peroxidase from spinach.
Ascorbate peroxidase (APX) (EC 1.11.1.11) activity was measured according to the method of Zhu et al.
For analysis of
ascorbate peroxidase (APX) and peroxidase (POD) activities, total soluble proteins were extracted from the leaves of plants with extraction buffer (for APX: 50 mM HEPES, pH 7.0, 0.1 mM EDTA; for POD: 100 mM potassium phosphate, pH 6.0), and protein concentrations were determined by the Bio-Rad protein assay kit.
Ascorbate peroxidase (APX, EC 1.11.1.11) activity was determined according to the method of Nakano and Asada [21].
Inactivation of
ascorbate peroxidase by thiols require hydrogen peroxide.
In the ascorbate- glutathione cycle, two key enzymes,
ascorbate peroxidase (APX) and guaiacol peroxidase (GP), are assumed to deal with the detoxification of reactive oxygen species in plants.
Expression of spinach
Ascorbate Peroxidase isoenzimes in response to oxidative stress.
(2005), catalase (Cakmark et al., 1993) and
ascorbate peroxidase (Krivosheeva et al., 1996) activities.
Plant defense systems to reduce the free oxygen in the cellular tissue and repair damaged tissue often can be in the role of antioxidant enzymes superoxide dismutase, catalase,
ascorbate peroxidase, glutathione reductase, glutathione peroxidase, dihydro ascorbat reductase [14].
Separate assays specific for
ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolic isozymes of
ascorbate peroxidase in plants.