To 5 mg of F-21-HS, 200 [micro]L of dimethyl
formamide, 200 [micro]L of dioxan and 100 [micro]L of distilled water containing 10 mg of N-hydroxysuccinimide and 20 mg of 1-ethyl-3-(3-dimethyl-amino-propyl) carbodiimide-HCL were added; reaction mixture was vortex-mixed and kept overnight at 4 [degrees]C.
Synthesis of a limited number of novel
formamide fungicides.
As probe liquids, double-distilled water, ethylene glycol, glycerol and
formamide were used, as purchased in the maximum obtainable purity.
A 2-[micro]L portion of the PCR product was diluted with 4 [micro]L of the SSCP buffer (475 mL/L
formamide, 0.5 g/L xylene cyanol, 0.2 g/L bromphenol blue, 5 mmol/L Tris, and 0.5 mmol/L EDTA).
Alcohol, tetrachloromethane, and N, N-dimethyl
formamide were all of analytic grade, purchased from Shanghai Chemical Reagent Co.
Final sample preparation was performed by adding 1 [micro]L of the post-PER to 9 [micro]L of
formamide. We loaded each sample on the ABI 310 genetic analyzer and analyzed the samples using Pop4 polymer, a 47-cm capillary column, and the ABI GeneScan E Run Module.
Compounds containing nitrogen, phosphorus or sulfur atoms, such as dimethyl
formamide (DMF), N,N-dimethyl aniline (DMA) and triphenyl phosphite (TPP), were reported to be capable of inhibiting the homopolymerization of MA and preventing cross linking/degradation which normally accompanies MA grafting on molten polymers.
We combined 1 [micro]L of PCR product diluted 1:10, 12 [micro]L of deionized
formamide, and 0.5 [micro]L of 6-carboxytetramethylrhodamine (TAMRA)-labeled size marker.