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    Try the export statement without the double quotes. Commented Dec 14, 2020 at 19:00
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    ls $HOME/Scripts/2bRAD_Edwards_Lab ? "the directory is not found" - please post the entire output. Bash couldn't have said that - it ignores PATHs which don't exist. Commented Dec 14, 2020 at 19:20
  • Please update the question with the exact command you run, the exact output you get and the output of some command to see this file, its permissions etc, like ls -al path/to/the/scripts/script_name.sh. Commented Dec 14, 2020 at 19:37
  • Note: This way you don't keep any previous PATH values. The usual way is to extend the existing PATH, whatever it is. Commented Dec 14, 2020 at 19:42
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    Thank you @thanasisp and Artem, I just added more information! I don't think I asked that clearly enough. Commented Dec 15, 2020 at 21:11